Human folliculin ELISA Kit | FLCN elisa kit

Human Folliculin, FLCN ELISA Kit

Cat. Num. AAA946456

• Gene Names: FLCN; BHD; FLCL
• Reactivity: Human
• Synonyms: folliculin; Human Folliculin; FLCN ELISA Kit; Human Folliculin (FLCN) ELISA kit; BHD; DKFZp547A118; FLCL; FLJ45004; FLJ99377; MGC17998; MGC23445; ; FLCN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FLCN. No significant cross-reactivity or interference between human FLCN and analogues was observed.
• Sequence Length: 342

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: The minimum detectable dose of human FLCN is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLCN elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FLCN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FLCN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FLCN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLCN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 21389403
• NCBI GeneID: 201163
• NCBI Accession #: NP_653207.1
• NCBI GenBank Nucleotide #: NM_144606.5
• UniProt Accession #: Q8NFG4; Q6ZRX1; Q96BD2; Q96BE4; A6NJJ8
• Molecular Weight: 64,473 Da
• NCBI Official Full Name: folliculin isoform 2
• NCBI Official Synonym Full Names: folliculin
• NCBI Official Symbol: FLCN
• NCBI Official Synonym Symbols: BHD; FLCL
• NCBI Protein Information: folliculin; birt-Hogg-Dube syndrome protein; BHD skin lesion fibrofolliculoma protein
• UniProt Protein Name: Folliculin
• Protein Family: Folliculin
• UniProt Gene Name: FLCN
• UniProt Synonym Gene Names: BHD
• UniProt Entry Name: FLCN_HUMAN

NCBI Description

This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FLCN: a protein that is expressed in most tissues and that has no significant homology to other proteins. Involved in nutrient sensing through the AMPK and mTOR signaling pathways. Regulates lysosome function by promoting the mTORC1-dependent sequestration of transcription factor EB (TFEB). Required for the recruitment of mTORC1 to lysosomes by Rag GTPases. Amino acid depletion leads to its recruitment to the surface of lysosomes where it directly binds RagA, an interaction that is promoted by FNIP1. May be a tumor suppressor. Loss of function mutations cause Birt-Hogg-Dube syndrome (BHD), a disease characterized by fibrofolliculomas (hair follicle tumors), and predispositions for renal carcinoma, pneumothorax, and pulmonary cysts. These symptoms are similar to those that occur in TSC or tuberous sclerosis complex, suggesting the involvement in the same pathways as TSC1. Belongs to the folliculin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasm; plasma membrane; midbody; nucleus

Molecular Function: protein binding; protein complex binding

Biological Process: intercellular junction assembly; negative regulation of TOR signaling pathway; regulation of protein amino acid phosphorylation; negative regulation of protein kinase B signaling cascade; TOR signaling pathway; positive regulation of cell adhesion; positive regulation of apoptosis; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; regulation of cytokinesis; regulation of TOR signaling pathway; negative regulation of Rho protein signal transduction; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; negative regulation of cell migration; positive regulation of TOR signaling pathway

Disease: Birt-hogg-dube Syndrome; Pneumothorax, Primary Spontaneous; Colorectal Cancer; Renal Cell Carcinoma, Nonpapillary