Rabbit anti-Mouse Fibrinogen Alpha (FGa) Polyclonal Antibody | anti-FGa antibody

Polyclonal Antibody to Fibrinogen Alpha (FGa)

Cat. Num. AAA2006768

• Gene Names: Fga; Fib
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Fibrinogen Alpha (FGa); Polyclonal Antibody; Polyclonal Antibody to Fibrinogen Alpha (FGa); anti-FGa antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against FGa. It has been selected for its ability to recognize FGa in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF- WGVF SEFGDSSSPA TRKEYHTGKA VTSKGDKELL IGKEKVTSSG TSTTHRSCSK TITKTVTGPD GRREVVKEVI TSDDGSDCGD ATELDISHSF SGSLDELSER HPDLSGFFDN HFGLISPNFK EFGSKTHSDS DILTNIEDPS SHVPEFSSSS KTSTVKKQVT KTYKMADEAG SEAHREGETR NTKRGRARAR PTRDCD
• Sequence Length: 644

• Applicable Applications for anti-FGa antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant FGa (Trp357~Asp556) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2051953
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

• NCBI GI #: 167555029
• NCBI GeneID: 14161
• NCBI Accession #: NP_001104518.1
• NCBI GenBank Nucleotide #: NM_001111048.2
• UniProt Accession #: E9PV24; Q99K47
• Molecular Weight: 61,326 Da
• NCBI Official Full Name: fibrinogen alpha chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: fibrinogen alpha chain
• NCBI Official Symbol: Fga
• NCBI Official Synonym Symbols: Fib
• NCBI Protein Information: fibrinogen alpha chain
• UniProt Protein Name: Fibrinogen alpha chain
• Protein Family: Fibrinogen
• UniProt Gene Name: Fga

NCBI Description

This gene encodes a subunit of the coagulation factor fibrinogen, which is a component of the blood clot. The encoded protein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mice lacking the encoded protein display bleeding in the peritoneal cavity, skin and soft tissues around joints immediately after birth, and are predisposed to spontaneous fatal abdominal hemorrhage as they grow. Pregnant mice lacking the encoded protein succumb to uterine bleeding during gestation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Nov 2015]

Uniprot Description

FGA: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGA are a cause of congenital afibrinogenemia (CAFBN). This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha- dysfibrinogenemias. Defects in FGA are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3 E3|3 36.96 cM

Cellular Component: blood microparticle; cell cortex; cell surface; cytoplasm; external side of plasma membrane; extracellular exosome; extracellular region; extracellular space; fibrinogen complex; rough endoplasmic reticulum

Molecular Function: cell adhesion molecule binding; metal ion binding; protein binding, bridging; receptor binding; structural molecule activity

Biological Process: adaptive immune response; blood coagulation; blood coagulation, common pathway; blood coagulation, fibrin clot formation; cell-matrix adhesion; cellular protein complex assembly; fibrinolysis; hemostasis; immune system process; induction of bacterial agglutination; innate immune response; negative regulation of endothelial cell apoptotic process; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; plasminogen activation; platelet activation; positive regulation of ERK1 and ERK2 cascade; positive regulation of exocytosis; positive regulation of heterotypic cell-cell adhesion; positive regulation of peptide hormone secretion; positive regulation of protein secretion; positive regulation of smooth muscle cell migration; positive regulation of vasoconstriction; protein complex assembly; protein polymerization; response to calcium ion; signal transduction