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Western Blot (WB) (Western Blot: Sample: Recombinant OGN, Mouse.)

Rabbit anti-Mouse Osteoglycin (OGN) Polyclonal Antibody | anti-OGN antibody

Polyclonal Antibody to Osteoglycin (OGN)

Gene Names
Ogn; OG; OIF; SLRR3A; mimecan; mimican; 3110079A16Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Osteoglycin (OGN); Polyclonal Antibody; Polyclonal Antibody to Osteoglycin (OGN); anti-OGN antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against OGN. It has been selected for its ability to recognize OGN in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.28mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-L RLPVLPPKLT LLNAKHNKIK SKGIKANTFK KLNKLSFLYL DHNDLESVPP NLPESLRVIH LQFNSISSLT DDTFCKANDT RYIRERIEEI RLEGNPIALG KHPNSFICLK RLPIGSYF
Sequence Length
298
Applicable Applications for anti-OGN antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant OGN (Leu180~Phe298) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2067569
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant OGN, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant OGN, Mouse.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,012 Da
NCBI Official Full Name
mimecan
NCBI Official Synonym Full Names
osteoglycin
NCBI Official Symbol
Ogn
NCBI Official Synonym Symbols
OG; OIF; SLRR3A; mimecan; mimican; 3110079A16Rik
NCBI Protein Information
mimecan
UniProt Protein Name
Mimecan
Protein Family
UniProt Gene Name
Ogn
UniProt Synonym Gene Names
Og

Uniprot Description

OGN: Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13|13 A5

Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: growth factor activity

Biological Process: axonogenesis

Research Articles on OGN

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Product Notes

The OGN ogn (Catalog #AAA2006014) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Osteoglycin (OGN) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Osteoglycin (OGN) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the OGN ogn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-L RLPVLPPKLT LLNAKHNKIK SKGIKANTFK KLNKLSFLYL DHNDLESVPP NLPESLRVIH LQFNSISSLT DDTFCKANDT RYIRERIEEI RLEGNPIALG KHPNSFICLK RLPIGSYF. It is sometimes possible for the material contained within the vial of "Osteoglycin (OGN), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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