Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Collagen Type VI Alpha 1 (COL6a1) Polyclonal Antibody | anti-COL6a1 antibody
Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)
Gene Names
Col6a1; Col6a-1; AI747156
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Collagen Type VI Alpha 1 (COL6a1); Polyclonal Antibody; Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1); anti-COL6a1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against COL6a1. It has been selected for its ability to recognize COL6a1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- NLVWNAGAL HYSDEVEIIR GLTRMPSGRD ELKASVDAVK YFGKGTYTDC AIKKGLEELL IGGSHLKENK YLIVVTDGHP LEGYKEPCGG LEDAVNEAKH LGIKVFSVAI TPDHLEPRLS IIATDHTYRR NF
MGHHHHHHSGSEF- NLVWNAGAL HYSDEVEIIR GLTRMPSGRD ELKASVDAVK YFGKGTYTDC AIKKGLEELL IGGSHLKENK YLIVVTDGHP LEGYKEPCGG LEDAVNEAKH LGIKVFSVAI TPDHLEPRLS IIATDHTYRR NF
Sequence Length
1028
Applicable Applications for anti-COL6a1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant COL6a1 (Asn82~Phe212) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2063622
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
108,489 Da
NCBI Official Full Name
collagen alpha-1(VI) chain
NCBI Official Synonym Full Names
collagen, type VI, alpha 1
NCBI Official Symbol
Col6a1
NCBI Official Synonym Symbols
Col6a-1; AI747156
NCBI Protein Information
collagen alpha-1(VI) chain
UniProt Protein Name
Collagen alpha-1(VI) chain
Protein Family
UniProt Gene Name
Col6a1
Uniprot Description
COL6A1: Collagen VI acts as a cell-binding protein. Defects in COL6A1 are a cause of Bethlem myopathy (BM). BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles. Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD); also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. Belongs to the type VI collagen family.
Protein type: Extracellular matrix; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10 C1|10 39.71 cM
Cellular Component: collagen; extracellular matrix; extracellular region; extracellular space; lysosomal membrane; membrane; protein complex; proteinaceous extracellular matrix; sarcolemma
Molecular Function: platelet-derived growth factor binding
Biological Process: cell adhesion; osteoblast differentiation
Research Articles on COL6a1
Similar Products
Product Notes
The COL6a1 col6a1 (Catalog #AAA2004192) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen Type VI Alpha 1 (COL6a1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the COL6a1 col6a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- NLVWNAGAL HYSDEVEIIR GLTRMPSGRD ELKASVDAVK YFGKGTYTDC AIKKGLEELL IGGSHLKENK YLIVVTDGHP LEGYKEPCGG LEDAVNEAKH LGIKVFSVAI TPDHLEPRLS IIATDHTYRR NF. It is sometimes possible for the material contained within the vial of "Collagen Type VI Alpha 1 (COL6a1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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