Rabbit FGD5 Polyclonal Antibody | anti-FGD5 antibody

FGD5 Antibody

Cat. Num. AAA9611764

• Gene Names: FGD5; ZFYVE23
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Horse, Sheep, Rabbit, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: FGD5; Polyclonal Antibody; FGD5 Antibody; FYVE; RhoGEF and PH domain containing protein 5; ZFYVE23; Zinc finger FYVE domain containing protein 23; anti-FGD5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Horse, Sheep, Rabbit, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FGD5 Antibody detects endogenous levels of FGD5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1462

• Applicable Applications for anti-FGD5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human FGD5.
• Tissue Specificity: Expressed in endothelial cells (at protein level).
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Endoplasmic Reticulum; Endosome; Cytoskeleton; Golgi Apparatus; Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using FGD5 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-FGD5 antibody

Description: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Mediates VEGF-induced CDC42 activation. May regulate proangiogenic action of VEGF in vascular endothelial cells, including network formation, directional movement and proliferation. May play a role in regulating the actin cytoskeleton and cell shape.

NCBI and Uniprot Product Information

• NCBI GI #: 194018497
• NCBI GeneID: 152273
• NCBI Accession #: NP_689749.3
• NCBI GenBank Nucleotide #: NP_689749.3
• UniProt Accession #: Q6ZNL6
• Molecular Weight: Observed: 160 kDa; Predicted: 160 kDa
• NCBI Official Full Name: FYVE, RhoGEF and PH domain-containing protein 5 isoform 1
• NCBI Official Synonym Full Names: FYVE, RhoGEF and PH domain containing 5
• NCBI Official Symbol: FGD5
• NCBI Official Synonym Symbols: ZFYVE23
• NCBI Protein Information: FYVE, RhoGEF and PH domain-containing protein 5
• UniProt Protein Name: FYVE, RhoGEF and PH domain-containing protein 5
• Protein Family: FYVE, RhoGEF and PH domain-containing protein
• UniProt Gene Name: FGD5
• UniProt Synonym Gene Names: ZFYVE23
• UniProt Entry Name: FGD5_HUMAN

Uniprot Description

FGD5: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Mediates VEGF-induced CDC42 activation. May regulate proangiogenic action of VEGF in vascular endothelial cells, including network formation, directional movement and proliferation. May play a role in regulating the actin cytoskeleton and cell shape. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: ruffle; Golgi apparatus; cytoskeleton; lamellipodium; endoplasmic reticulum; early endosome; cytoplasm

Molecular Function: small GTPase binding; protein binding; Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; metal ion binding

Biological Process: regulation of cell shape; filopodium formation; cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity