SDS-PAGE
CAMK1G / CLICK III / CaMKIgamma recombinant protein
Recombinant Human CAMK1G / CLICK III / CaMKIgamma Protein (His & GST tag)
Gene Names
CAMK1G; VWS1; CLICK3; CLICKIII; dJ272L16.1
Purity
> 85 % as determined by SDS-PAGE
Synonyms
CAMK1G / CLICK III / CaMKIgamma; Recombinant Human CAMK1G / CLICK III / CaMKIgamma Protein (His & GST tag); RP1-272L16.2; CLICKIII; VWS1; dJ272L16.1; CAMK1G / CLICK III / CaMKIgamma recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
> 85 % as determined by SDS-PAGE
Form/Format
Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.3mM DTT
Sequence Length
476
Application Notes
The recombinant human CAMK1G/GST chimera consists of 713 amino acids and has a calculated molecular mass of 81 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Met
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for CAMK1G / CLICK III / CaMKIgamma recombinant protein
Background: Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKIgamma is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIgamma membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIgamma critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIgamma specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIgamma may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.
Description: A DNA sequence encoding the human CAMK1G isoform 1 (Q96NX5-1) (Met 1-Met 476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Description: A DNA sequence encoding the human CAMK1G isoform 1 (Q96NX5-1) (Met 1-Met 476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,486 Da
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type 1G
NCBI Official Synonym Full Names
calcium/calmodulin dependent protein kinase IG
NCBI Official Symbol
CAMK1G
NCBI Official Synonym Symbols
VWS1; CLICK3; CLICKIII; dJ272L16.1
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type 1G
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type 1G
UniProt Gene Name
CAMK1G
UniProt Synonym Gene Names
CLICK3; VWS1; CaM kinase IG; CaM-KI gamma; CaMKI gamma; CaMKIG; CLICK III
NCBI Description
This gene encodes a protein similar to calcium/calmodulin dependent protein kinase, however, its exact function is not known. [provided by RefSeq, Jul 2008]
Uniprot Description
CAMK1G: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: CAMK group; CAMK1 family; EC 2.7.11.17; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 1q32.2
Cellular Component: intracellular
Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity
Biological Process: peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation
Research Articles on CAMK1G / CLICK III / CaMKIgamma
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Product Notes
The CAMK1G / CLICK III / CaMKIgamma camk1g (Catalog #AAA2545033) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human CAMK1G/GST chimera consists of 713 amino acids and has a calculated molecular mass of 81 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the CAMK1G / CLICK III / CaMKIgamma camk1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMK1G / CLICK III / CaMKIgamma, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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