Rabbit anti-Human FEZ2 Polyclonal Antibody | anti-FEZ2 antibody

FEZ2, NT (FEZ2, Fasciculation and elongation protein zeta-2, Zygin II, Zygin-2) (Azide free) (HRP)

Cat. Num. AAA6298850

• Gene Names: FEZ2; HUM3CL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FEZ2; Polyclonal Antibody; NT (FEZ2; Fasciculation and elongation protein zeta-2; Zygin II; Zygin-2) (Azide free) (HRP); Zygin-2; anti-FEZ2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FEZ2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FEZ2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FEZ2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FEZ2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the FEZ2 antibody detected the FEZ2 protein (arrow).)

Related Product Information for anti-FEZ2 antibody

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin.

Product Categories/Family for anti-FEZ2 antibody

Antibodies; Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010). Assmann, E.M., et al. J. Biol. Chem. 281(15):9869-9881(2006). Hillier, L.W., et al. Nature 434(7034):724-731(2005). Fujita, T., et al. Biochem. Biophys. Res. Commun. 313(3):738-744(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 110349754
• NCBI GeneID: 9637
• NCBI Accession #: NP_005093.2
• NCBI GenBank Nucleotide #: NM_005102.2
• UniProt Accession #: Q9UHY8; Q5EBN3; Q76LN0; Q99690
• Molecular Weight: 42,613 Da
• NCBI Official Full Name: fasciculation and elongation protein zeta-2 isoform 1
• NCBI Official Synonym Full Names: fasciculation and elongation protein zeta 2 (zygin II)
• NCBI Official Symbol: FEZ2
• NCBI Official Synonym Symbols: HUM3CL
• NCBI Protein Information: fasciculation and elongation protein zeta-2; zygin 2; zygin-2; zygin II; pre-T/NK cell associated protein (3Cl)
• UniProt Protein Name: Fasciculation and elongation protein zeta-2
• Protein Family: Fasciculation and elongation protein
• UniProt Gene Name: FEZ2
• UniProt Entry Name: FEZ2_HUMAN

NCBI Description

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin. [provided by RefSeq, Jul 2008]

Uniprot Description

FEZ2: Involved in axonal outgrowth and fasciculation. Belongs to the zygin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 2p21

Molecular Function: protein binding

Biological Process: axon guidance; nervous system development; signal transduction