Human Reticulocalbin 1 ELISA Kit | RCN1 elisa kit

Human Reticulocalbin 1 ELISA Kit

Cat. Num. AAA762480

• Gene Names: RCN1; RCN; RCAL; PIG20; HEL-S-84
• Reactivity: Human
• Synonyms: Reticulocalbin 1; Human Reticulocalbin 1 ELISA Kit; RCN1 (RCN)/Reticulocalbin-1; RCN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RCN1. No significant cross-reactivity or interference between RCN1 and analogues was observed.
• Sequence Length: 331

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for RCN1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506455
• NCBI GeneID: 5954
• NCBI Accession #: NP_002892.1
• NCBI GenBank Nucleotide #: NM_002901.2
• Molecular Weight: 33,026 Da
• NCBI Official Full Name: reticulocalbin-1
• NCBI Official Synonym Full Names: reticulocalbin 1
• NCBI Official Symbol: RCN1
• NCBI Official Synonym Symbols: RCN; RCAL; PIG20; HEL-S-84
• NCBI Protein Information: reticulocalbin-1
• UniProt Protein Name: Reticulocalbin-1
• Protein Family: Reticulocalbin
• UniProt Gene Name: RCN1
• UniProt Synonym Gene Names: RCN
• UniProt Entry Name: RCN1_HUMAN

NCBI Description

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009]

Uniprot Description

RCN1: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: calcium ion binding; protein binding

Biological Process: camera-type eye development; in utero embryonic development