Rabbit anti-Human BAAT Polyclonal Antibody | anti-BAAT antibody

BAAT Antibody (N-Term)

Cat. Num. AAA9217189

• Gene Names: BAAT; BAT; BACAT
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: BAAT; Polyclonal Antibody; BAAT Antibody (N-Term); Bile acid-CoA:amino acid N-acyltransferase; BACAT; BAT; 2.3.1.65; Glycine N-choloyltransferase; Long-chain fatty-acyl-CoA hydrolase; 3.1.2.2; anti-BAAT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This BAAT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 107-148 amino acids from human BAAT.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 418

• Applicable Applications for anti-BAAT antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:2000
• Subtitle: Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Cytoplasm.
• Tissue Location: Expressed in liver, gallbladder mucosa and pancreas.
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-BAAT Antibody (N-Term) at 1:2000 dilutionLane 1: human liver lysateLane 2: HepG2 whole cell lysateLane 3: HL-60 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-BAAT antibody

Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.

Product Categories/Family for anti-BAAT antibody

Primary Antibodies; Cancer; Metabolism; Signal Transduction

References

Falany C.N.,et al.J. Biol. Chem. 269:19375-19379 (1994). Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Humphray S.J.,et al.Nature 429:369-374 (2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Johnson M.R.,et al.J. Biol. Chem. 266:10227-10233 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 189083866
• NCBI GeneID: 570
• NCBI Accession #: NP_001121082.1
• NCBI GenBank Nucleotide #: NM_001127610.1
• UniProt Accession #: Q14032; Q3B7W9; Q96L31
• Molecular Weight: 46299 Da
• NCBI Official Full Name: bile acid-CoA:amino acid N-acyltransferase
• NCBI Official Synonym Full Names: bile acid-CoA:amino acid N-acyltransferase
• NCBI Official Symbol: BAAT
• NCBI Official Synonym Symbols: BAT; BACAT
• NCBI Protein Information: bile acid-CoA:amino acid N-acyltransferase
• UniProt Protein Name: Bile acid-CoA:amino acid N-acyltransferase
• Protein Family: Bile acid-CoA:amino acid N-acyltransferase
• UniProt Gene Name: BAAT
• UniProt Synonym Gene Names: BAT
• UniProt Entry Name: BAAT_HUMAN

NCBI Description

The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BAAT: Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs. Defects in BAAT are involved in familial hypercholanemia (FHCA). FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. Belongs to the C/M/P thioester hydrolase family.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; Other Amino Acids Metabolism - taurine and hypotaurine; EC 3.1.2.2; EC 2.3.1.65; Hydrolase; Transferase; Lipid Metabolism - primary bile acid biosynthesis

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytosol; peroxisomal matrix; peroxisome

Molecular Function: glycine N-choloyltransferase activity; N-acyltransferase activity; palmitoyl-CoA hydrolase activity; protein binding; receptor binding; transferase activity, transferring acyl groups

Biological Process: acyl-CoA metabolic process; bile acid biosynthetic process; bile acid metabolic process; fatty acid metabolic process; glycine metabolic process; liver development; organ regeneration; taurine metabolic process

Disease: Hypercholanemia, Familial