Rabbit FEZ1 Polyclonal Antibody | anti-FEZ1 antibody

FEZ1 Antibody

Cat. Num. AAA9630670

• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: FEZ1; Polyclonal Antibody; FEZ1 Antibody; Fasciculation and elongation protein zeta 1; Fasciculation and elongation protein zeta-1; Fez 1; Fez; FEZ1_HUMAN; Fezl; Zygin 1; Zygin I; Zygin-1; Zygin1; ZyginI; anti-FEZ1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: FEZ1 Antibody detects endogenous levels of FEZ1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-FEZ1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human FEZ1.
• Conjugation: Unconjugated
• Similarity: Belongs to the zygin family.
• Post Translational Modifications: Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination.Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26.
• Subunit Structure: Homodimer; disulfide-linked. May form heterodimers with FEZ2. Interacts with the NH2-terminal variable region (V1) of PKC zeta and weakly with that of PKC epsilon (By similarity). Interacts with UBE4B. Interacts with SAP30L. Interacts with SCOC and ULK1; SCOC interferes with ULK1-binding to FEZ1. Directly interacts with SCOC and UVRAG. Stabilizes the interaction between SCOC and UVRAG during amino acid starvation.
• Tissue Specificity: Mainly expressed in brain.
• Function: May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.
• Note: Colocalizes with both, alpha- and gamma-tubulin. Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-FEZ1 antibody

Cytoplasm; Cytoskeleton; Microtubule organizing center; Centrosome; Cell membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 13431526
• NCBI GeneID: 9638
• UniProt Accession #: Q99689; O00679; O00728; Q6IBI7
• Molecular Weight: 12,074 Da
• NCBI Official Full Name: Fasciculation and elongation protein zeta-1
• NCBI Official Synonym Full Names: fasciculation and elongation protein zeta 1 (zygin I)
• NCBI Official Symbol: FEZ1
• NCBI Protein Information: fasciculation and elongation protein zeta-1; zygin I; zygin-1
• UniProt Protein Name: Fasciculation and elongation protein zeta-1
• Protein Family: Fasciculation and elongation protein
• UniProt Gene Name: FEZ1
• UniProt Entry Name: FEZ1_HUMAN

NCBI Description

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

FEZ1: May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules. Belongs to the zygin family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: microtubule cytoskeleton; microtubule; centrosome; mitochondrion; axon; dendrite; cytoplasm; plasma membrane

Molecular Function: protein binding; gamma-tubulin binding; protein kinase C binding; protein N-terminus binding

Biological Process: establishment of mitochondrion localization; axon guidance; nervous system development; transport; cell adhesion