Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nodal homolog (NODAL) Standard Curve.)
Mouse Nodal Homolog (NODAL) ELISA Kit | NODAL elisa kit
Mouse Nodal Homolog (NODAL) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nodal homolog (NODAL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nodal homolog (NODAL) Standard Curve.)
Background: Nodal is a protein that in humans is encoded by the NODAL gene. It is a member of the TGF beta superfamily. Like many other members of this superfamily, it is involved in cell differentiation. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of left-right axial structures in early embryonic development. The left-right asymmetry in the body of vertebrates is established by a process that uses the Nodal and Lefty proteins; Nodal is expressed in the left side of the organism in early development. An ortholog of Nodal was recently found in snails and was shown to be involved in left-right asymmetry as well.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. Homozygous knockout mice for this gene exhibit early embryonic lethality, while expression of a hypomorphic allele results in defects in anteroposterior and left-right patterning. [provided by RefSeq, Aug 2016]
Uniprot Description
NODAL: Essential for mesoderm formation and axial patterning during embryonic development. Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. Belongs to the TGF-beta family.
Protein type: Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10 B4|10 32.21 cM
Cellular Component: extracellular region; extracellular space
Molecular Function: cytokine activity; protein binding; receptor agonist activity; transforming growth factor beta receptor binding
Biological Process: anatomical structure formation; anterior/posterior axis specification; anterior/posterior pattern formation; axial mesodermal cell fate specification; BMP signaling pathway; brain development; cell fate commitment; cell migration; cell migration involved in gastrulation; determination of left/right symmetry; digestive tract morphogenesis; embryonic cranial skeleton morphogenesis; embryonic heart tube development; embryonic pattern specification; embryonic placenta development; embryonic process involved in female pregnancy; endoderm development; floor plate morphogenesis; formation of anatomical boundary; gastrulation; gastrulation with mouth forming second; heart development; heart looping; in utero embryonic development; liver development; lung development; maternal placenta development; maternal process involved in parturition; mesendoderm development; mesoderm formation; negative regulation of cell differentiation; negative regulation of transcription from RNA polymerase II promoter; nervous system development; neural fold formation; placenta development; polarity specification of proximal/distal axis; positive regulation of activin receptor signaling pathway; positive regulation of angiogenesis; positive regulation of caspase activity; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of epithelial cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; regulation of gastrulation; regulation of MAPKKK cascade; regulation of signal transduction; repression of premature neural plate formation; stem cell maintenance; tissue morphogenesis; transforming growth factor beta receptor signaling pathway; trophectodermal cell differentiation; trophectodermal cellular morphogenesis; vasculature development
Research Articles on NODAL
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Product Notes
The Mouse NODAL nodal (Catalog #AAA9717988) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717988 ELISA Kit recognizes Mouse NODAL. It is sometimes possible for the material contained within the vial of "Nodal Homolog (NODAL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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