FOXO3A Polyclonal Antibody | anti-FOXO3A antibody

Anti-FOXO3A Rabbit pAb

Cat. Num. AAA477132

• Gene Names: FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: FOXO3A; Polyclonal Antibody; Anti-FOXO3A Rabbit pAb; AF6q21; AF6q21 protein; DKFZp781A0677; FKHR2; FKHRL 1; FKHRL1; FKHRL1P2; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1; Forkhead box O3; Forkhead box O3A; Forkhead box protein O3; Forkhead box protein O3A; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1; Forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma like 1; Forkhead in rhabdomyosarcoma-like 1; FOX O3A; FOXO2; foxo3; FOXO3_HUMAN; MGC12739; MGC31925; anti-FOXO3A antibody

• Host: Rabbit; Source: Human
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: 10mM PBS with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 673

• Applicable Applications for anti-FOXO3A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human FOXO3A around the phosphorylation site of serine 253.
• Sensitivity: This antibody detects endogenous levels of FOXO3A and does not cross-react with related proteins.
• Sequence Similarities: Contains 1 fork-head DNA-binding domain.
• Subunit Structure: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4/MST1, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. Interacts with PIM1. Interacts with DDIT3/CHOP.
• Subcellular Location: Cytoplasm; cytosol. Nucleus. Note: Translocates to the nucleus upon oxidative stress and in the absence of survival factors.

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells treated with using Serum. Left: Using FOXO3A (Ab-253) antibody.Right: Using the same antibody preincubated with synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FOXO3A (Ab-253) antibody.)

Related Product Information for anti-FOXO3A antibody

Entrez Summary: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 4503739
• NCBI GeneID: 2309
• NCBI Accession #: NP_001446.1
• NCBI GenBank Nucleotide #: NM_001455.3
• UniProt Accession #: O43524; O15171; Q5T2I7; Q9BZ04; B4DVZ6; E1P5E6
• Molecular Weight: 71
• NCBI Official Full Name: forkhead box protein O3
• NCBI Official Synonym Full Names: forkhead box O3
• NCBI Official Symbol: FOXO3
• NCBI Official Synonym Symbols: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• NCBI Protein Information: forkhead box protein O3
• UniProt Protein Name: Forkhead box protein O3
• UniProt Gene Name: FOXO3

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.

Protein type: Autophagy; DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; chromatin DNA binding; DNA binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity

Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of apoptosis; positive regulation of erythrocyte differentiation; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of translation; tumor necrosis factor-mediated signaling pathway