Rabbit anti-Human HMGN2 Polyclonal Antibody | anti-HMGN2 antibody

HMGN2, NT (HMGN2, HMG17, Non-histone chromosomal protein HMG-17, High mobility group nucleosome-binding domain-containing protein 2) (PE)

Cat. Num. AAA6307340

• Gene Names: HMGN2; HMG17
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HMGN2; Polyclonal Antibody; NT (HMGN2; HMG17; Non-histone chromosomal protein HMG-17; High mobility group nucleosome-binding domain-containing protein 2) (PE); High mobility group nucleosome-binding domain-containing protein 2; anti-HMGN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HMGN2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HMGN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HMGN2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HMGN2 antibody detected the HMGN2 protein (arrow).)

Related Product Information for anti-HMGN2 antibody

HMGN2 binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation (By similarity).

Product Categories/Family for anti-HMGN2 antibody

Antibodies; Histones

References

Rattner, B.P., et al. Mol. Cell 34(5):620-626(2009). Ueda, T., et al. Mol. Cell. Biol. 28(9):2872-2883(2008). Cherukuri, S., et al. Mol. Biol. Cell 19(5):1816-1824(2008). Wu, C., et al. Proteomics 7(11):1775-1785(2007). Porkka, K., et al. Proc. Natl. Acad. Sci. U.S.A. 99(11):7444-7449(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 5031749
• NCBI GeneID: 3151
• NCBI Accession #: NP_005508.1
• NCBI GenBank Nucleotide #: NM_005517.3
• UniProt Accession #: P05204; Q0VGD5; Q6FGI5; Q96C64
• Molecular Weight: 9,393 Da
• NCBI Official Full Name: non-histone chromosomal protein HMG-17
• NCBI Official Synonym Full Names: high mobility group nucleosomal binding domain 2
• NCBI Official Symbol: HMGN2
• NCBI Official Synonym Symbols: HMG17
• NCBI Protein Information: non-histone chromosomal protein HMG-17; high mobility group protein N2; nonhistone chromosomal protein HMG-17; high-mobility group nucleosomal binding domain 2; high-mobility group (nonhistone chromosomal) protein 17; high mobility group nucleosome-bindin
• UniProt Protein Name: Non-histone chromosomal protein HMG-17
• Protein Family: Non-histone chromosomal protein
• UniProt Gene Name: HMGN2
• UniProt Synonym Gene Names: HMG17
• UniProt Entry Name: HMGN2_HUMAN

NCBI Description

The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi. [provided by RefSeq, Oct 2014]

Uniprot Description

HMGN2: Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Belongs to the HMGN family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: cytoplasm; nucleus; chromatin

Molecular Function: nucleosomal DNA binding