Western Blot (WB)
(Staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat FBXW2 Polyclonal Antibody | anti-FBXW2 antibody
Goat anti-FBXW2 Antibody
Immunohistochemistry: In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml. An anonymous customer found positive results in IHC on Mouse kidney tissue. An anonymous customer found positive results in IHC on Rat kidney tissue.
Western Blot: Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48. 0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5ug/ml. A stronger band of unknown identity was also consistently observed at appr.30kDa - both bands were successfully blocked by incubation with the immunising peptide.
Western Blot (WB)
(Staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats. [provided by RefSeq, Jul 2008]
Uniprot Description
FBXW2: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 9q34
Molecular Function: protein binding; ubiquitin-protein ligase activity
Biological Process: protein modification process; protein ubiquitination; proteolysis
Research Articles on FBXW2
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Product Notes
The FBXW2 fbxw2 (Catalog #AAA421205) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FBXW2 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FBXW2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml. An anonymous customer found positive results in IHC on Mouse kidney tissue. An anonymous customer found positive results in IHC on Rat kidney tissue. Western Blot: Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48. 0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5ug/ml. A stronger band of unknown identity was also consistently observed at appr.30kDa - both bands were successfully blocked by incubation with the immunising peptide. Researchers should empirically determine the suitability of the FBXW2 fbxw2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KRGSSFLAGE HPG. It is sometimes possible for the material contained within the vial of "FBXW2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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