Porcine Hexokinase ELISA Kit | HK elisa kit

Porcine Hexokinase ELISA Kit

Cat. Num. AAA736244

• Gene Names: HKL3; hexokinase-like 3; HKL3; T28I19.120; T28I19_120
• Reactivity: Porcine
• Synonyms: Hexokinase; Porcine Hexokinase ELISA Kit; HK elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 U/L.
• Intended Uses: This HK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine HK. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HK elisa kit

Principle of the Assay: HK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HK antibody and an HK-HRP conjugate. The assay sample and buffer are incubated together with HK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HK concentration since HK from samples and HK-HRP conjugate compete for the anti-HK antibody binding site. Since the number of sites is limited, as more sites are occupied by HK from the sample, fewer sites are left to bind HK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HK concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15235722
• NCBI GeneID: 829940
• NCBI Accession #: NP_195497.1
• NCBI GenBank Nucleotide #: NM_119945.1
• Molecular Weight: 54,241 Da
• NCBI Official Full Name: hexokinase-like 3
• NCBI Official Symbol: HKL3
• NCBI Official Synonym Symbols: hexokinase-like 3; HKL3; T28I19.120; T28I19_120
• NCBI Protein Information: hexokinase-like 3
• UniProt Protein Name: Probable hexokinase-like 2 protein
• Protein Family: Hexokinase
• UniProt Entry Name: HXKL2_ARATH

NCBI Description

Encodes a putative hexokinase.