Rabbit anti-Mouse FBXO8 Polyclonal Antibody | anti-FBXO8 antibody

FBXO8, ID (FBXO8, FBS, FBX8, F-box only protein 8, F-box/SEC7 protein FBS) (Azide free) (HRP)

Cat. Num. AAA6298498

• Gene Names: FBXO8; FBS; DC10; FBX8
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBXO8; Polyclonal Antibody; ID (FBXO8; FBS; FBX8; F-box only protein 8; F-box/SEC7 protein FBS) (Azide free) (HRP); F-box/SEC7 protein FBS; anti-FBXO8 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FBXO8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FBXO8 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 167-195 amino acids selected from the Central region of human FBXO8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXO8 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the FBXO8 antibody detected the FBXO8 protein (arrow).)

Product Categories/Family for anti-FBXO8 antibody

Antibodies; Ubiquitin Ligases

References

Cronin, S., et al. Eur. J. Hum. Genet. 17(2):213-218(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Ilyin, G.P., et al. Genomics 67(1):40-47(2000). Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 48928044
• NCBI GeneID: 26269
• NCBI Accession #: NP_036312.2
• NCBI GenBank Nucleotide #: NM_012180.2
• UniProt Accession #: Q9NRD0; Q6UWN4; Q8IWE1; Q9NRP5; Q9UKC4; B2RB40; D3DP41; G5E9Z0
• Molecular Weight: 32,426 Da
• NCBI Official Full Name: F-box only protein 8
• NCBI Official Synonym Full Names: F-box protein 8
• NCBI Official Symbol: FBXO8
• NCBI Official Synonym Symbols: FBS; DC10; FBX8
• NCBI Protein Information: F-box only protein 8
• UniProt Protein Name: F-box only protein 8
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO8
• UniProt Synonym Gene Names: FBS; FBX8
• UniProt Entry Name: FBX8_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It contains a C-terminal amino acid sequence that bears a significant similarity with a portion of yeast Sec7p, a critical regulator of vesicular protein transport. This human protein may interact with ADP-ribosylation factor(s)(ARFs) and exhibit ARF-GEF (guanine nucleotide exchange factor) activity. [provided by RefSeq, Jul 2008]