Rabbit anti-Human POLR2B Polyclonal Antibody | anti-POLR2B antibody

POLR2B, ID (POLR2B, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II 140kD polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II subunit 2, RNA polymerase II subunit B2) (PE)

Cat. Num. AAA6335696

• Gene Names: POLR2B; RPB2; POL2RB; hsRPB2; hRPB140
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR2B; Polyclonal Antibody; ID (POLR2B; DNA-directed RNA polymerase II subunit RPB2; DNA-directed RNA polymerase II 140kD polypeptide; DNA-directed RNA polymerase II subunit B; RNA polymerase II subunit 2; RNA polymerase II subunit B2) (PE); RNA polymerase II subunit B2; anti-POLR2B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-POLR2B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 796-826 amino acids from the Central region of human POLR2B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLR2B Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR2B antibody detected the POLR2B protein (arrow).)

Related Product Information for anti-POLR2B antibody

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq].

Product Categories/Family for anti-POLR2B antibody

Antibodies; Enzymes; Polymerase

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Michiels, S., et al. Carcinogenesis 30(5):763-768(2009). Tu, S., et al. Nat. Struct. Mol. Biol. 15(4):419-421(2008). Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008). Yamaguchi, Y., et al. Genes Cells 12(7):863-875(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4505941
• NCBI GeneID: 5431
• NCBI Accession #: NP_000929.1
• NCBI GenBank Nucleotide #: NM_000938.1
• UniProt Accession #: P30876; Q8IZ61; A8K1A8; AAB23139
• Molecular Weight: 133,897 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB2
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
• NCBI Official Symbol: POLR2B
• NCBI Official Synonym Symbols: RPB2; POL2RB; hsRPB2; hRPB140
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB2; RNA polymerase II subunit 2; RNA polymerase II subunit B2; DNA-directed RNA polymerase II subunit B; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA directed RNA polymerase II 140 kDa polypeptide, RNA p
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB2
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR2B
• UniProt Entry Name: RPB2_HUMAN