Rabbit FBXO11 Polyclonal Antibody | anti-FBXO11 antibody

FBXO11 antibody

Cat. Num. AAA9406224

• Gene Names: FBXO11; UBR6; VIT1; FBX11; PRMT9; UG063H01
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: FBXO11; Polyclonal Antibody; FBXO11 antibody; UBR6; VIT1; FBX11; PRMT9; UG063H01; anti-FBXO11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total FBXO11 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 927

• Applicable Applications for anti-FBXO11 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:20 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human FBXO11.
• Target Name: FBXO11
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FBXO11 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cell using FBXO11 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-FBXO11 antibody

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

Product Categories/Family for anti-FBXO11 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 298231105
• NCBI GeneID: 80204
• NCBI Accession #: NP_001177203.1
• NCBI GenBank Nucleotide #: NM_001190274.1
• UniProt Accession #: Q86XK2; Q52ZP1; Q53EP7; Q53RT5; Q8IXG3; Q96E90; Q9H6V8; Q9H9L1; Q9NR14; Q9UFK1; Q9UHI1; A1L491
• Molecular Weight: 106,864 Da
• NCBI Official Full Name: F-box only protein 11 isoform 4
• NCBI Official Synonym Full Names: F-box protein 11
• NCBI Official Symbol: FBXO11
• NCBI Official Synonym Symbols: UBR6; VIT1; FBX11; PRMT9; UG063H01
• NCBI Protein Information: F-box only protein 11
• UniProt Protein Name: F-box only protein 11
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO11
• UniProt Synonym Gene Names: FBX11; PRMT9; VIT1; VIT-1
• UniProt Entry Name: FBX11_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

PRMT9: Substrate recognition component of the a (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of TP53. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cytoplasm; nucleolus; chromosome; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein-arginine N-methyltransferase activity; zinc ion binding; ubiquitin-protein ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; sensory perception of sound; protein modification process; protein ubiquitination; peptidyl-arginine N-methylation