Western Blot (WB)
(Western Blot analysis of HEK-293 cells using OCT4 Monoclonal Antibody at dilution of 1:4000)
Mouse anti-Human, Mouse Oct-3/4 Monoclonal Antibody | anti-Oct-3/4 antibody
Oct-3/4 Monoclonal Antibody
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Protein G purification
Synonyms
Oct-3/4; Monoclonal Antibody; Oct-3/4 Monoclonal Antibody; Octamer binding transcription factor 4; MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF 3; OTF 4; OTF-3; OTF3; OTF4; PO5F1; POU class 5 homeobox 1; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain; class 5; transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1; anti-Oct-3/4 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6645
Purity/Purification
Protein G purification
Concentration
1mg/mL (varies by lot)
Sequence Length
265
Applicable Applications for anti-Oct-3/4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-1:10000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
Fusion protein of OCT4
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK-293 cells using OCT4 Monoclonal Antibody at dilution of 1:4000)
Western Blot (WB)
(Western Blot analysis of HEK-293 cells using OCT4 Monoclonal Antibody at dilution of 1:4000)
Related Product Information for anti-Oct-3/4 antibody
Expression of the OCT4 transcription factor is associated with an undifferentiated phenotype of human and mouse embryonic stem cells and germ cells. OCT4 (Octamer-4) is a homeodomain transcription factor of the POU family (POU5F1) involved in regulation of pluripotency during normal development and is detectable in embryonic stem (ES) and germ cells. Its expression is associated with an undifferentiated phenotype and tumors. In fact, gene knockdown of OCT4 promotes differentiation, thereby demonstrating a role for this factor in human ES cell self-renewal. As such, it is frequently used as a marker for undifferentiated ES cells or iPS (induced pluripotent stem) cells. OCT4 is one of the four key transcription factors (OCT4, SOX2, c-MYC, and KLF4) used to reprogram mouse and human fibroblasts to a pluripotent state as well as generate patient-specific iPS cells.
Product Categories/Family for anti-Oct-3/4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 39kDa
Observed: 50-52kDa
Observed: 50-52kDa
NCBI Official Full Name
POU domain, class 5, transcription factor 1 isoform 3
NCBI Official Synonym Full Names
POU class 5 homeobox 1
NCBI Official Symbol
POU5F1
NCBI Official Synonym Symbols
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
NCBI Protein Information
POU domain, class 5, transcription factor 1
UniProt Protein Name
POU domain, class 5, transcription factor 1
UniProt Gene Name
POU5F1
UniProt Synonym Gene Names
OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
NCBI Description
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]
Uniprot Description
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Research Articles on Oct-3/4
Similar Products
Product Notes
The Oct-3/4 pou5f1 (Catalog #AAA2540220) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Oct-3/4 Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Oct-3/4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-1:10000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the Oct-3/4 pou5f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Oct-3/4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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