Rabbit anti-Human ID3 Polyclonal Antibody | anti-ID3 antibody

ID3, CT (DNA-binding Protein Inhibitor ID-3, Class B Basic Helix-loop-helix Protein 25, bHLHb25, Helix-loop-helix Protein HEIR-1, ID-like Protein Inhibitor HLH 1R21, Inhibitor of DNA Binding 3, 1R21, BHLHB25, HEIR1)

Cat. Num. AAA641989

• Gene Names: ID3; HEIR-1; bHLHb25
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ID3; Polyclonal Antibody; CT (DNA-binding Protein Inhibitor ID-3; Class B Basic Helix-loop-helix Protein 25; bHLHb25; Helix-loop-helix Protein HEIR-1; ID-like Protein Inhibitor HLH 1R21; Inhibitor of DNA Binding 3; 1R21; BHLHB25; HEIR1); Anti -ID3; anti-ID3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human ID3.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.

• Applicable Applications for anti-ID3 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human ID3 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Human breast carcinoma stained with ID3)

Immunohistochemistry (IHC)

(Human Adrenal (formalin-fixed, paraffin-embedded) stained with ID3 antibody at 10ug/ml followed by biotinylated goat rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with ID3 antibody at 10ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Lung (formalin-fixed, paraffin-embedded) stained with ID3 antibody at 10ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-ID3 antibody

Id3, a 14kD protein that is a member of the Id family of helix-loop-helix proteins, is believed to be a dominant negative regulator of bHLH proteins involved in cell differentiation and implicated in the control of cell growth, cellular differentiation, apoptosis and carcinogenesis

Product Categories/Family for anti-ID3 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 313213
• NCBI GeneID: 3399
• UniProt Accession #: Q02535; O75641; A8K1T8
• Molecular Weight: 12,999 Da
• NCBI Official Full Name: Id3
• NCBI Official Synonym Full Names: inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
• NCBI Official Symbol: ID3
• NCBI Official Synonym Symbols: HEIR-1; bHLHb25
• NCBI Protein Information: DNA-binding protein inhibitor ID-3; helix-loop-helix protein HEIR-1; ID-like protein inhibitor HLH 1R21; class B basic helix-loop-helix protein 25
• UniProt Protein Name: DNA-binding protein inhibitor ID-3
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID3
• UniProt Synonym Gene Names: 1R21; BHLHB25; HEIR1; bHLHb25
• UniProt Entry Name: ID3_HUMAN

NCBI Description

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the encoded protein lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it interacts. [provided by RefSeq, Aug 2011]

Uniprot Description

ID3: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. Induced by phorbol 12-myristate 13-acetate (PMA). Interacts with COPS5 and COPS7A. Homodimer, and heterodimer with other HLH proteins.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.13-p36.12

Cellular Component: microtubule cytoskeleton; nucleoplasm; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein domain specific binding; protein binding; transcription corepressor activity; transcription factor binding; transcription factor activity

Biological Process: circadian rhythm; muscle development; central nervous system development; transcription, DNA-dependent; positive regulation of apoptosis; regulation of cell cycle; multicellular organismal development; heart development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; notochord development; odontogenesis; neuron differentiation; epithelial cell differentiation; response to wounding; negative regulation of osteoblast differentiation; negative regulation of myoblast differentiation; negative regulation of transcription, DNA-dependent; metanephros development; regulation of DNA replication