Western Blot (WB)
(Western blot analysis of CALML3 using K562 whole cell lysates)
Rabbit CALML3 Polyclonal Antibody | anti-CALML3 antibody
CALML3 Antibody
Gene Names
CALML3; CLP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CALML3; Polyclonal Antibody; CALML3 Antibody; CALL3_HUMAN; Calmodulin like 3; Calmodulin related protein NB 1; Calmodulin-like protein 3; Calmodulin-related protein NB-1; CaM like protein; CaM-like protein; CLP; OTTHUMP00000019004; anti-CALML3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CALML3 antibody detects endogenous levels of total CALML3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
149
Applicable Applications for anti-CALML3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human CALML3
Tissue Specificity
Expressed in normal mammary, prostate, cervical, and epidermal tissues. It is greatly reduced or undetectable in transformed cells.
Predicted Cross Reactivity
Horse, Chicken
Similarity
Horse (80%), Chicken (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CALML3 using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of CALML3 using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using CALML3 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using CALML3 Antibody.)
Immunohistochemistry (IHC)
(MBS9609632 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609632 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CALML3 antibody
Function: May function as a specific light chain of unconventional myosin-10 (MYO10), also enhances MYO10 translation, possibly by acting as a chaperone for the emerging MYO10 heavy chain protein. May compete with calmodulin by binding, with different affinities, to cellular substrates.
Subunit Structure: Interacts with MYO10, the interaction is calcium-dependent and essential for MYO10 function in filopodial extension.
Similarity: Belongs to the calmodulin family.
Subunit Structure: Interacts with MYO10, the interaction is calcium-dependent and essential for MYO10 function in filopodial extension.
Similarity: Belongs to the calmodulin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 17 kDa
Predicted: 17 kDa
Predicted: 17 kDa
NCBI Official Full Name
calmodulin-like protein 3
NCBI Official Synonym Full Names
calmodulin like 3
NCBI Official Symbol
CALML3
NCBI Official Synonym Symbols
CLP
NCBI Protein Information
calmodulin-like protein 3
UniProt Protein Name
Calmodulin-like protein 3
Protein Family
UniProt Gene Name
CALML3
UniProt Synonym Gene Names
CLP
Uniprot Description
May function as a specific light chain of unconventional myosin-10 (MYO10), also enhances MYO10 translation, possibly by acting as a chaperone for the emerging MYO10 heavy chain protein. May compete with calmodulin by binding, with different affinities, to cellular substrates.
Research Articles on CALML3
Similar Products
Product Notes
The CALML3 calml3 (Catalog #AAA9609632) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CALML3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CALML3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the CALML3 calml3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CALML3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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