Rabbit anti-Human, Mouse FBXL18 Polyclonal Antibody | anti-FBXL18 antibody

FBXL18 Polyclonal Antibody

Cat. Num. AAA3014178

• Gene Names: FBXL18; Fbl18
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified.
• Synonyms: FBXL18; Polyclonal Antibody; FBXL18 Polyclonal Antibody; F-box/LRR-repeat protein 18; F-box and leucine-rich repeat protein 18; FBL18; anti-FBXL18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of FBXL18 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 718

• Applicable Applications for anti-FBXL18 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Synthetic peptide corresponding to human FBXL18 aa 750 to C terminus.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of FBXL18 on PC-3M cell lysate using anti-FBXL18 antibody at 1:500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-FBXL18 antibody. Counter stained with hematoxylin.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of A549 cells with FBXL18 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-FBXL18 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemicalparaffin-embedded mouse liveranti-FBXL18 antibody. Counterhematoxylin.)

Related Product Information for anti-FBXL18 antibody

FBL18 (F-box and leucine-rich repeat protein 18), also known as FBXL18, is an 805 amino acid protein that contains an F-box near its N-terminus, followed by several leucine-rich repeats and a transmembrane domain at the C-terminus. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune response, signaling cascades and developmental processes) through the targeting of proteins, such as cyclins and cyclin-dependent kinase inhibitors (CDKNs), for degradation by the proteasome after ubiquitination.

NCBI and Uniprot Product Information

• NCBI GI #: 157426875
• NCBI GeneID: 80028
• NCBI Accession #: NP_079239.3
• NCBI GenBank Nucleotide #: NP_079239.3
• UniProt Accession #: Q96ME1
• Molecular Weight: ~ 40, 88kDa
• NCBI Official Full Name: F-box/LRR-repeat protein 18 isoform 2
• NCBI Official Synonym Full Names: F-box and leucine rich repeat protein 18
• NCBI Official Symbol: FBXL18
• NCBI Official Synonym Symbols: Fbl18
• NCBI Protein Information: F-box/LRR-repeat protein 18
• UniProt Protein Name: F-box/LRR-repeat protein 18
• Protein Family: F-box/LRR-repeat protein
• UniProt Gene Name: FBXL18
• UniProt Synonym Gene Names: FBL18
• UniProt Entry Name: FXL18_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of proteins that contain an approximately 40-amino acid F-box motif. This motif is important for interaction with SKP1 and for targeting some proteins for degradation. The encoded protein has been shown to control the cellular level of FBXL7, a protein that induces mitotic arrest, by targeting it for polyubiquitylation and proteasomal degradation. Members of the F-box protein family, such as FBXL18, are characterized by an approximately 40-amino acid F-box motif. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains. [provided by RefSeq, Mar 2016]