Rabbit Fat Free Polyclonal Antibody | anti-C11orf2 antibody

Fat Free Antibody

Cat. Num. AAA150363

• Gene Names: VPS51; FFR; ANG2; ANG3; C11orf2; C11orf3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Fat Free Antibody is affinity chromatography purified via peptide column.
• Synonyms: Fat Free; Polyclonal Antibody; Fat Free Antibody; FFR; ANG2; ANG3; C11orf2; C11orf3; PP5382; Vacuolar protein sorting-associated protein 51 homolog; Another new gene 2 protein; chromosome 11 open reading frame 2; anti-C11orf2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.
• Purity/Purification: Fat Free Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 782

• Applicable Applications for anti-C11orf2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Fat Free antibody can be used for detection of Fat Free by Western blot at 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free.
• Buffer: Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Fat Free antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.)

Related Product Information for anti-C11orf2 antibody

Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.

NCBI and Uniprot Product Information

• NCBI GI #: 8393009
• NCBI GeneID: 738
• NCBI Accession #: NP_037397
• NCBI GenBank Nucleotide #: NM_013265.3
• UniProt Accession #: Q9UID3; Q6PJV5; Q7L8A6; Q8WZ35; Q96DF4; Q96GR3
• Molecular Weight: 86 kDa
• NCBI Official Full Name: vacuolar protein sorting-associated protein 51 homolog
• NCBI Official Synonym Full Names: vacuolar protein sorting 51 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS51
• NCBI Official Synonym Symbols: FFR; ANG2; ANG3; C11orf2; C11orf3
• NCBI Protein Information: vacuolar protein sorting-associated protein 51 homolog; protein fat-free homolog; another new gene 2 protein
• UniProt Protein Name: Vacuolar protein sorting-associated protein 51 homolog
• UniProt Gene Name: VPS51
• UniProt Synonym Gene Names: ANG2; C11orf2; C11orf3; FFR
• UniProt Entry Name: VPS51_HUMAN

NCBI Description

This gene encodes a member of the vacuolar protein sorting-associated protein 51 family. The encoded protein is a component of the Golgi-associated retrograde protein complex which acts as a tethering factor for carriers in retrograde transport from the early and late endosomes to the trans-Golgi network. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Uniprot Description

FFR: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi networkl (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN. Belongs to the VPS51 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: Golgi apparatus; integral to membrane

Molecular Function: protein binding

Biological Process: protein transport; autophagy; lipid transport; retrograde transport, endosome to Golgi