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Western Blot (WB) (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela and K562 cell lysates, Primary antibody: 121396(VPS18 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 50 seconds)

Rabbit anti-Human VPS18 Polyclonal Antibody | anti-VPS18 antibody

VPS18 Rabbit pAb

Gene Names
VPS18; PEP3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
VPS18; Polyclonal Antibody; VPS18 Rabbit pAb; anti-VPS18 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-VPS18 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
Immunogen
Fusion protein of human VPS18
Conjugation
Unconjugated
Molecular Function
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
Modification
Unmodified
Cell Position
Cytoplasmic vesicle, Endosome, Lysosome, Membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela and K562 cell lysates, Primary antibody: 121396(VPS18 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 50 seconds)

Western Blot (WB) (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela and K562 cell lysates, Primary antibody: 121396(VPS18 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 50 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 121396(VPS18 Antibody) at dilution 1/35. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 121396(VPS18 Antibody) at dilution 1/35. (Original magnification: ×200))
Product Categories/Family for anti-VPS18 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
110,186 Da
NCBI Official Full Name
Homo sapiens vacuolar protein sorting 18 homolog (S. cerevisiae), mRNA
NCBI Official Synonym Full Names
VPS18, CORVET/HOPS core subunit
NCBI Official Symbol
VPS18
NCBI Official Synonym Symbols
PEP3
NCBI Protein Information
vacuolar protein sorting-associated protein 18 homolog
UniProt Protein Name
Vacuolar protein sorting-associated protein 18 homolog
UniProt Gene Name
VPS18
UniProt Synonym Gene Names
KIAA1475
UniProt Entry Name
VPS18_HUMAN

NCBI Description

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq, Jul 2008]

Research Articles on VPS18

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Product Notes

The VPS18 vps18 (Catalog #AAA4754340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS18 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VPS18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the VPS18 vps18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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