Rabbit anti-Human C11orf2 / Fat-Free Polyclonal Antibody | anti-VPS51 antibody

Rabbit Polyclonal to Human C11orf2 / Fat-Free

Cat. Num. AAA248914

• Gene Names: VPS51; FFR; ANG2; ANG3; C11orf2; C11orf3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: C11orf2 / Fat-Free; Polyclonal Antibody; Rabbit Polyclonal to Human C11orf2 / Fat-Free; Anti-C11orf2 / Fat-Free Antibody (C-Terminus) IHC-plus; ANG2; Another new gene 2 protein; C11orf3; FFR; Fat-free homolog; Protein fat-free homolog; ANG3; Human C11orf2; Fat-Free; anti-VPS51 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human C11orf2 / Fat-Free. At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 782

• Applicable Applications for anti-VPS51 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: Fat-free antibody can be used for detection of Fat-free by Western blot at 1-2 ug/mL.
• Target Species: Human
• Immunogen Description: An 18 amino acid peptide near the carboxy terminus of human Fat-free.
• Immunogen Type: Synthetic peptide
• Immunogen: C11orf2 / Fat-Free antibody was raised against an 18 amino acid peptide near the carboxy terminus of human Fat-free.
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-C11orf2 / Fat-Free antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 8393009
• NCBI GeneID: 738
• NCBI Accession #: NP_037397.2
• NCBI GenBank Nucleotide #: NM_013265.3
• UniProt Accession #: Q9UID3; Q6PJV5; Q7L8A6; Q8WZ35; Q96DF4; Q96GR3
• Molecular Weight: 72,655 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 51 homolog
• NCBI Official Synonym Full Names: vacuolar protein sorting 51 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS51
• NCBI Official Synonym Symbols: FFR; ANG2; ANG3; C11orf2; C11orf3
• NCBI Protein Information: vacuolar protein sorting-associated protein 51 homolog; protein fat-free homolog; another new gene 2 protein
• UniProt Protein Name: Vacuolar protein sorting-associated protein 51 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS51
• UniProt Synonym Gene Names: ANG2; C11orf2; C11orf3; FFR
• UniProt Entry Name: VPS51_HUMAN

NCBI Description

This gene encodes a member of the vacuolar protein sorting-associated protein 51 family. The encoded protein is a component of the Golgi-associated retrograde protein complex which acts as a tethering factor for carriers in retrograde transport from the early and late endosomes to the trans-Golgi network. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Uniprot Description

FFR: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi networkl (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN. Belongs to the VPS51 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: Golgi apparatus; integral to membrane

Molecular Function: protein binding

Biological Process: protein transport; autophagy; lipid transport; retrograde transport, endosome to Golgi