Rabbit FAS-L Polyclonal Antibody | anti-FAS-L antibody

FAS-L (A19) Antibody

Cat. Num. AAA440039

• Gene Names: Fasl; gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• Reactivity: Mouse, rat, human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: FAS-L; Polyclonal Antibody; FAS-L (A19) Antibody; anti-FAS-L antibody

• Host: Rabbit
• Reactivity: Mouse, rat, human
• Clonality: Polyclonal
• Specificity: Mouse, rat and human FAS-L
• Form/Format: 200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.200 mg/ml (varies by lot)
• Sequence Length: 279

• Applicable Applications for anti-FAS-L antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Origin: FAS-L (A19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping near the amino terminus of rat FAS-L.
• Immunogen: Synthetic peptide mapping near the amino terminal domain of rat FAS-L.
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Related Product Information for anti-FAS-L antibody

FAS/APO-1 (CD95) is an important member of the tumor necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by FAS ligand or an anti-FAS cross-linking antibody, triggers activation of the caspase cascade (1). Functional impairment of the FAS/FAS-L system is associated with the development and progression of malignancies (2). FAS gene mutations have been suggested to have a role in testicular germ cell tumors (3). Tumor cells frequently exhibit de novo expression of FAS-ligand (FAS-L), which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells (8). The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the FAS/FAS-ligand system (7). FAS is believed to be involved in various autoimmune diseases including, ulcerative colitis, Graves disease, and rheumatoid arthritis (5,7). FAS expression on gastric epithelial cells in patients infected with H. Pylori is responsible for the accelerated apoptosis of the cells (4). Serum FAS-L concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension (6).

References

1. Lin P, Bush JA, Cheung KJ Jr, Li G. Tissue-specific regulation of Fas/APO-1/CD95 expression by p53. Int J Oncol 2002 Aug;21(2):261-4.2. Redondo P, Solano T, VAzquez B, Bauza A, Idoate M. Fas and Fas ligand: expression and soluble circulating levels in cutaneous malignant melanoma. Br J Dermatol 2002 Jul;147(1):80-63. Takayama H, Takakuwa T, Tsujimoto Y, Tani Y, Nonomura N, Okuyama A, Nagata S, Aozasa K. Frequent fas gene mutations in testicular germ cell tumors. Am J Pathology 2002 Aug; 161(2):635-41.4. Hasumi K, Tanaka K, Saitoh S, Takagi A, Miwa T, Mine T, Koga Y. Roles of tumor necrosis factor-alpha-receptor type 1 and Fas in the Helicobacter pylori-induced apoptosis of gastric epithelial cells. J Gastroenterol Hepatol 2002 Jun;17(6):651-658.5. Yukawa M, Iizuka M, Horie Y, Yoneyama K, Shirasaka T, Itou H, Komatsu M, Fukushima T, Watanabe S.Systemic and local evidence of increased Fas-mediated apoptosis in ulcerative colitis. Int J Colorectal Dis 2002 Mar;17(2):70-66. Okura T, Watanabe S, Jiang Y, Nakamura M, Takata Y, Yang ZH, Kohara K, Kitami Y, Hiwada K. Soluble Fas ligand and atherosclerosis in hypertensive patients. J Hypertens 2002 May;20(5):895-87. Hara H, Sato R, Ban Y. Accelerated production of nucleosome in cultured human mononuclear cells in untreated Graves' disease. Endocr J 2002 Apr;49(2):189-948. Reichmann E.The biological role of the Fas/FasL system during tumor formation and progression. Semin Cancer Biol 2002 Aug;12(4):309-15

NCBI and Uniprot Product Information

• NCBI GI #: 31127301
• NCBI GeneID: 14103
• UniProt Accession #: P41047; Q61217; Q9R1F2
• Molecular Weight: 8,040 Da
• NCBI Official Full Name: Fasl protein
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: Fasl
• NCBI Official Synonym Symbols: gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas antigen ligand; generalized lymphoproliferative disease
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• UniProt Gene Name: Faslg
• UniProt Synonym Gene Names: Apt1lg1; Cd95l; Fasl; gld; Tnfsf6; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
• UniProt Entry Name: TNFL6_MOUSE

Uniprot Description

FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Membrane protein, integral

Cellular Component: extracellular space; lysosome; cytoplasmic membrane-bound vesicle; integral to membrane; extracellular region; caveola; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; external side of plasma membrane

Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: caspase activation; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; positive regulation of neuron apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; retinal cell programmed cell death; immune response