Hamster CD178 Monoclonal Antibody | anti-CD178 antibody

HAMSTER ANTI MOUSE CD178:RPE

Cat. Num. AAA219545

• Gene Names: Fasl; gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD178; Monoclonal Antibody; HAMSTER ANTI MOUSE CD178:RPE; anti-CD178 antibody

• Host: Hamster
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: MFL4
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Concentration: IgG concentration 0.1mg/ml (varies by lot)
• Sequence Length: 279

• Applicable Applications for anti-CD178 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.; Flow Cytometry: Minimum Dilution: Neat
• Reconstitution: Reconstitute with 1ml distilled water ; Perservative Stabilisers
• Immunogen: B6 mouse FasL/BHK cells; Buffer Solution
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of mouse CD178 transfected cells with Hamster anti Mouse CD178:FITC (MBS219358))

Testing Data

(Staining of mouse CD178 transfected cells with Hamster anti Mouse CD178:Alexa Fluor ?647 (MBS219438A647))

Testing Data

(Staining of mouse CD178 transfected cells with Hamster anti Mouse CD178:RPE)

Testing Data

(Staining of mouse CD178 transfected cells with Hamster anti Mouse CD178:Alexa Fluor ?488 (MBS219438A488))

Related Product Information for anti-CD178 antibody

Hamster anti Mouse CD178 antibody, clone MFL4 recognizes both mouse and rat CD178, otherwise known as Fas Ligand/ CD95L, a 40kDa type II transmembrane glycoprotein and member of the TNF/NGF superfamily, expressed by activated T cells and NK cells, which can be induced on a variety of cells by radiation, heat shock, chemotherapeutic agents and viral infection. CD178 acts as a key effector of cytotoxicity and in the regulation of immune responses. The binding of CD178 to its receptor CD95 (Fas), induces Fas-mediated apoptosis of target cells, and may be involved in the induction of peripheral tolerance and neutrophil chemotaxis. The binding of decoy receptor 3 (DcR3) to CD178 has been shown to inhibit CD178-mediated apoptosis. Hamster anti Mouse CD178 antibody, clone MFL4 is reported to block CD178/CD95 induced apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 6753818
• NCBI GeneID: 14103
• NCBI Accession #: NP_034307.1
• NCBI GenBank Nucleotide #: NM_010177.4
• UniProt Accession #: P41047; Q61217; Q9R1F2
• Molecular Weight: 8,040 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: Fasl
• NCBI Official Synonym Symbols: gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas antigen ligand; generalized lymphoproliferative disease
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• UniProt Gene Name: Faslg
• UniProt Synonym Gene Names: Apt1lg1; Cd95l; Fasl; gld; Tnfsf6; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
• UniProt Entry Name: TNFL6_MOUSE

Uniprot Description

FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral; Cytokine

Cellular Component: extracellular space; lysosome; cytoplasmic membrane-bound vesicle; extracellular region; integral to membrane; caveola; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; external side of plasma membrane

Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: caspase activation; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; negative regulation of transcription from RNA polymerase II promoter; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; induction of apoptosis via death domain receptors; regulation of transcription, DNA-dependent; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of neuron apoptosis; positive regulation of cell proliferation; immune response; retinal cell programmed cell death