Rabbit FANCL Polyclonal Antibody | anti-FANCL antibody

FANCL Antibody

Cat. Num. AAA9609793

• Gene Names: FANCL; POG; PHF9; FAAP43
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: FANCL; Polyclonal Antibody; FANCL Antibody; E3 ubiquitin-protein ligase FANCL; FAAP43; Fancl; FANCL_HUMAN; Fanconi anemia complementation group L; Fanconi anemia group L protein; Fanconi anemia-associated polypeptide of 43 kDa; FLJ10335; PHD finger protein 9; PHF9; POG; Ubiquitin ligase protein FANCL; anti-FANCL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FANCL antibody detects endogenous levels of total FANCL
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 380

• Applicable Applications for anti-FANCL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human FANCL
• Subcellular Location: Cytoplasm. Nucleus.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Bovine (100%), Horse (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%), Xenopus (89%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis FANCL using LOVO whole cell lysates)

Related Product Information for anti-FANCL antibody

Function: Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway (PubMed:12973351, PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:24389026). Also mediates monoubiquitination of FANCI (PubMed:19589784). May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
Subunit Structure: Interacts with GGN (By similarity). Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM (PubMed:12973351, PubMed:15502827, PubMed:16116422, PubMed:22343915). The complex is not found in FA patients. In complex with FANCF, FANCA and FANCG, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins (PubMed:18550849). Interacts with FANCI (PubMed:21775430). Directly interacts (via the RING-type zinc finger) with UBE2T and UBE2W (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:21775430, PubMed:24389026).
Post-translational Modifications: The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.
Similarity: The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.

NCBI and Uniprot Product Information

• NCBI GI #: 167860138
• NCBI GeneID: 55120
• NCBI Accession #: NP_001108108.1
• NCBI GenBank Nucleotide #: NM_001114636.1
• UniProt Accession #: Q9NW38; Q6GU60
• Molecular Weight: Observed: 43 kDa; Predicted: 43 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase FANCL isoform 1
• NCBI Official Synonym Full Names: FA complementation group L
• NCBI Official Symbol: FANCL
• NCBI Official Synonym Symbols: POG; PHF9; FAAP43
• NCBI Protein Information: E3 ubiquitin-protein ligase FANCL
• UniProt Protein Name: E3 ubiquitin-protein ligase FANCL
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: FANCL
• UniProt Synonym Gene Names: PHF9; FAAP43

NCBI Description

This gene encodes a ubiquitin ligase that is a member of the Fanconi anemia complementation group (FANC). Members of this group are related by their assembly into a common nuclear protein complex rather than by sequence similarity. This gene encodes the protein for complementation group L that mediates monoubiquitination of FANCD2 as well as FANCI. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2018]

Uniprot Description

Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway (PubMed:12973351, PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:24389026). Also mediates monoubiquitination of FANCI (PubMed:19589784). May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.