Mouse RAB3D Monoclonal Antibody | anti-RAB3D antibody

RAB3D Antibody: RPE

Cat. Num. AAA8005943

• Gene Names: RAB3D; GOV; D2-2; RAB16; RAD3D
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Protein G Purified
• Synonyms: RAB3D; Monoclonal Antibody; RAB3D Antibody: RPE; Mouse Anti-Human RAB3D Monoclonal IgG1; RAB3D Antibody; Clone 5E9: RPE; Member RAS Oncogene Family antibody; D2-2 antibody; GOV antibody; RAB16 antibody; RAD3D antibody; Ras-Related Protein Rab-3D antibody; anti-RAB3D antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5E9
• Specificity: Detects ~24kDa. Does not show cross reactivity to RAB3A, RAB3B, or RAB3C.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RAB3D antibody: Western Blot (WB)
• Application Notes: WB: 1:1000; A 1:1000 dilution was sufficient for detection of RAB3D in 10ug of A549, T-47D cells, mouse and rat brain, kidney by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary antibody.
• Immunogen: Full length recombinant RAB3D protein
• Immunogen Species: Human
• Conjugation: RPE
• Cellular Localization: Cell membrane, Membrane
• Preparation and Storage: Store at -20 degree C; Shipping: Blue Ice

Western Blot (WB)

(Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9 . Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D.. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody . Courtesy of: Dario Alessi Lab, University of Dundee.)

Western Blot (WB)

(Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9 . Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney.. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

Related Product Information for anti-RAB3D antibody

Rab3D is a small exocytic GTPase involved in protein transport. It is required for efficient bone resorption by osteoclasts (1) and associates with a subset of the trans-golgi network (2).

Product Categories/Family for anti-RAB3D antibody

Antibodies; Cancer; Cell Signaling; Cell Signaling; Autophagy| Golgi Proteins; Alzheimer's Disease; Growth Factors; Membrane Trafficking Proteins; Neurodegeneration; Neuroscience; Parkinson's Disease; Protein Trafficking

References

1. Zhu, S. et al. (2016) Sci Rep. 6:379632. Pavlos, N. J. et al. (2005) Mol Cell Biol. 25(12): 5253–5269

NCBI and Uniprot Product Information

• NCBI GI #: 4759000
• NCBI GeneID: 9545
• NCBI Accession #: NP_004274
• NCBI GenBank Nucleotide #: NM_004283.3
• UniProt Accession #: O95716
• Molecular Weight: 26.4 kDa (239aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: ras-related protein Rab-3D
• NCBI Official Synonym Full Names: RAB3D, member RAS oncogene family
• NCBI Official Symbol: RAB3D
• NCBI Official Synonym Symbols: GOV; D2-2; RAB16; RAD3D
• NCBI Protein Information: ras-related protein Rab-3D
• UniProt Protein Name: Ras-related protein Rab-3D
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB3D
• UniProt Synonym Gene Names: GOV; RAB16
• UniProt Entry Name: RAB3D_HUMAN

Uniprot Description

RAB3D: Protein transport. Probably involved in regulated exocytosis. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: synaptic vesicle; transport vesicle; mitochondrion; secretory granule membrane; plasma membrane; endosome

Molecular Function: GTPase activity; GTPase binding; GDP binding; GTP binding; myosin V binding

Biological Process: regulation of exocytosis; intracellular protein transport; synaptic vesicle exocytosis; protein secretion; peptidyl-cysteine methylation; Rab protein signal transduction; vesicle docking during exocytosis