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Typical Testing Data/Standard Curve (for reference only)

Human NAIP ELISA Kit | NAIP elisa kit

Human NAIP (Neuronal Apoptosis Inhibitory Protein) ELISA Kit

Gene Names
NAIP; BIRC1; NLRB1; psiNAIP
Reactivity
Human
Synonyms
NAIP; Human NAIP (Neuronal Apoptosis Inhibitory Protein) ELISA Kit; NAIP elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This kit recognizes Human NAIP in samples. No significant cross-reactivity or interference between Human NAIP and analogues was observed.
Sequence Length
1347
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.16-10ng/mL
Sensitivity
0.1ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NAIP were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NAIP were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NAIP elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human NAIP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NAIP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NAIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NAIP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NAIP. You can calculate the concentration of Human NAIP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
141,329 Da
NCBI Official Full Name
NAIP protein
NCBI Official Synonym Full Names
NLR family, apoptosis inhibitory protein
NCBI Official Symbol
NAIP
NCBI Official Synonym Symbols
BIRC1; NLRB1; psiNAIP
NCBI Protein Information
baculoviral IAP repeat-containing protein 1; neuronal apoptosis inhibitory protein; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domain containing 1; psi neuronal apoptosis inhibitory protein
UniProt Protein Name
Baculoviral IAP repeat-containing protein 1
UniProt Gene Name
NAIP
UniProt Synonym Gene Names
BIRC1
UniProt Entry Name
BIRC1_HUMAN

NCBI Description

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This copy of the gene is full length; additional copies with truncations and internal deletions are also present in this region of chromosome 5q13. It is thought that this gene is a modifier of spinal muscular atrophy caused by mutations in a neighboring gene, SMN1. The protein encoded by this gene contains regions of homology to two baculovirus inhibitor of apoptosis proteins, and it is able to suppress apoptosis induced by various signals. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BIRC1: Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor- neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: spindle microtubule; basolateral plasma membrane; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ATP binding

Biological Process: nervous system development; protein ubiquitination; regulation of signal transduction; innate immune response; negative regulation of caspase activity; negative regulation of neuron apoptosis; inflammatory response; negative regulation of apoptosis

Research Articles on NAIP

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Product Notes

The Human NAIP naip (Catalog #AAA2506880) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2506880 ELISA Kit recognizes Human NAIP. It is sometimes possible for the material contained within the vial of "NAIP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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