Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunD (Phospho-Ser255) Antibody. The picture on the right is blocked with the phospho peptide.)
Rabbit JunD Polyclonal Antibody | anti-JunD antibody
JunD (Phospho-Ser255) Antibody
Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
JunD; Polyclonal Antibody; JunD (Phospho-Ser255) Antibody; anti-JunD antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
JunD (Phospho-Ser255) Antibody detects endogenous levels of JunD only when phosphorylated at Ser255.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
347
Applicable Applications for anti-JunD antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
IHC: 1:50~1:100
ELISA: 1:20000
Target Modification
Phospho
Modification Sites
H:S255; M:S249; R:S249
Immunogen
The antiserum was produced against synthesized peptide derived from human JunD around the phosphorylation site of Ser255.
Immunogen Range
222-271
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunD (Phospho-Ser255) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunD (Phospho-Ser255) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from 293 cells treated with Forskolin, using JunD (Phospho-Ser255) Antibody. The lane on the left is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from 293 cells treated with Forskolin, using JunD (Phospho-Ser255) Antibody. The lane on the left is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35 kDa
NCBI Official Full Name
transcription factor jun-D isoform deltaJunD
NCBI Official Synonym Full Names
JunD proto-oncogene, AP-1 transcription factor subunit
NCBI Official Symbol
JUND
NCBI Official Synonym Symbols
AP-1
NCBI Protein Information
transcription factor jun-D
UniProt Protein Name
Transcription factor jun-D
Protein Family
UniProt Gene Name
JUND
NCBI Description
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013]
Uniprot Description
Transcription factor binding AP-1 sites.
Research Articles on JunD
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Product Notes
The JunD jund (Catalog #AAA9503081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JunD (Phospho-Ser255) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JunD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000. Researchers should empirically determine the suitability of the JunD jund for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JunD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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