Western Blot (WB)
(Western blot (WB) analysis of FADD polyclonal antibody at 1:5000 dilutionLane1:Pancl whole cell lysate(40ug)Lane2:HEK293T whole cell lysate(40ug)Lane3:Jurkat whole cell lysate(40ug))
Rabbit anti-Human FADD Polyclonal Antibody | anti-FADD antibody
FADD polyclonal antibody
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
Synonyms
FADD; Polyclonal Antibody; FADD polyclonal antibody; FAS-associated death domain protein; FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; Protein FADD; GIG3; anti-FADD antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
FADD polyclonal antibody detects endogenous levels of FADD protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.
Applicable Applications for anti-FADD antibody
Western Blot (WB)
Application Notes
WB: 1:2000~1:5000
Immunogen
Synthetic peptide, corresponding to Human FADD.
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of FADD polyclonal antibody at 1:5000 dilutionLane1:Pancl whole cell lysate(40ug)Lane2:HEK293T whole cell lysate(40ug)Lane3:Jurkat whole cell lysate(40ug))
Western Blot (WB)
(Western blot (WB) analysis of FADD polyclonal antibody at 1:5000 dilutionLane1:Pancl whole cell lysate(40ug)Lane2:HEK293T whole cell lysate(40ug)Lane3:Jurkat whole cell lysate(40ug))
Related Product Information for anti-FADD antibody
Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8. FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent and may be related to its regulatory role in embryonic development and cell cycle progression.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~30kDa
Protein Family
Similar Products
Product Notes
The FADD (Catalog #AAA3014604) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FADD polyclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FADD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:2000~1:5000. Researchers should empirically determine the suitability of the FADD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FADD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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