Human ATP-citrate synthase ELISA Kit | ACLY elisa kit

Human ATP-citrate synthase ELISA Kit

Cat. Num. AAA765522

• Gene Names: ACLY; ACL; ATPCL; CLATP
• Reactivity: Human
• Synonyms: ATP-citrate synthase; Human ATP-citrate synthase ELISA Kit; ACLY/ATP-citrate synthase/ATP-citrate (pro-S-)-lyase/ACL/Citrate cleavage enzyme/ATPCL/CLATP; ACLY elisa kit

• Reactivity: Human
• Sequence Length: 1145

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.56-100IU/ml
• Sensitivity: 0.938IU/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ACLY elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ACLY antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ACLY antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ACLY amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ACLY can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 740086852
• NCBI GeneID: 47
• NCBI Accession #: NP_001290204.1
• NCBI GenBank Nucleotide #: NM_001303275.1
• Molecular Weight: 91,099 Da
• NCBI Official Full Name: ATP-citrate synthase isoform 4
• NCBI Official Synonym Full Names: ATP citrate lyase
• NCBI Official Symbol: ACLY
• NCBI Official Synonym Symbols: ACL; ATPCL; CLATP
• NCBI Protein Information: ATP-citrate synthase
• UniProt Protein Name: ATP-citrate synthase
• Protein Family: ATP-citrate synthase
• UniProt Gene Name: ACLY
• UniProt Synonym Gene Names: ACL
• UniProt Entry Name: ACLY_HUMAN

NCBI Description

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.3.8; Carbohydrate Metabolism - citrate (TCA) cycle; Transferase; Lyase

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; plasma membrane

Molecular Function: ATP binding; ATP citrate synthase activity; cofactor binding; metal ion binding; protein binding

Biological Process: acetyl-CoA biosynthetic process; cellular lipid metabolic process; cholesterol biosynthetic process; citrate metabolic process; energy reserve metabolic process; fatty acid biosynthetic process; lipid biosynthetic process; oxaloacetate metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process