Rabbit anti-Human FADD Polyclonal Antibody | anti-FADD antibody

FADD, phosphorylated (Ser194)(Fas-Associated Death Domain Protein, MORT1)

Cat. Num. AAA628537

• Gene Names: FADD; GIG3; MORT1; MGC8528
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: FADD; Polyclonal Antibody; phosphorylated (Ser194)(Fas-Associated Death Domain Protein; MORT1); Anti -FADD; anti-FADD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of human FADD protein only when phosphorylated at Ser194.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-FADD antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Immunocytochemistry and Western Blot.; Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunocytochemistry (ABC): 1:200
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser194 of human FADD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat and HeLa cells, hydroxyurea-treated (4 mM, 20 hours) or nocodazole-treated (1 ug/ml, 20 hours), using Phospho-FADD Ser194 Antibody (Human Specific) (upper) or control FADD Antibody (Human Specific) #2782 (lower).)

Immunocytochemistry (ICC)

(Immunocytochemical staining of HeLa cells, hydroxyurea-treated (A, B) or nocodazole-treated (C, D), using Phospho-FADD (Ser194) Antibody (Human Specific) (A, C) or the same antibody preincubated with the antigenic peptide (B, D).)

Related Product Information for anti-FADD antibody

Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase- 8 (1,2). FADD has a C-terminal death domain interacting with the cytoplasmic tail of the membrane receptor and an N-terminal death effector domain interacting with caspase-8. Clustering of the receptors upon stimulation brings FADD together further to oligomerize caspase-8 and activate the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194 while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle dependent (3) and may be related to its regulatory role in embryonic development and the cell cycle process (4,5)

Product Categories/Family for anti-FADD antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 48145593
• NCBI GeneID: 8772
• UniProt Accession #: Q13158; Q14866; Q6IBR4; Q6LCB0; Q6LCG1
• Molecular Weight: 23,279 Da
• NCBI Official Full Name: FADD
• NCBI Official Synonym Full Names: Fas (TNFRSF6)-associated via death domain
• NCBI Official Symbol: FADD
• NCBI Official Synonym Symbols: GIG3; MORT1; MGC8528
• NCBI Protein Information: protein FADD; OTTHUMP00000235551; growth-inhibiting gene 3 protein; mediator of receptor induced toxicity; mediator of receptor-induced toxicity; Fas-associating protein with death domain; Fas-associating death domain-containing protein
• UniProt Protein Name: Protein FADD
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: FADD
• UniProt Synonym Gene Names: MORT1
• UniProt Entry Name: FADD_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq]

Uniprot Description

FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: neuron projection; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; protease binding; death receptor binding; protein complex binding; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: viral reproduction; positive regulation of T cell mediated cytotoxicity; apoptosis; positive regulation of proteolysis; protein heterooligomerization; positive regulation of apoptosis; T cell differentiation in the thymus; toll-like receptor 3 signaling pathway; positive regulation of activated T cell proliferation; positive regulation of interleukin-8 production; T cell homeostasis; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of adaptive immune response; spleen development; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; thymus development; MyD88-independent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor production; lymph node development; positive regulation of interferon-gamma production; induction of apoptosis via death domain receptors; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter

Disease: Infections, Recurrent, With Encephalopathy, Hepatic Dysfunction, And Cardiovascular Malformations