Rabbit FABP7 / BLBP / MRG Polyclonal Antibody | anti-FABP7 antibody

Rabbit Polyclonal to Human FABP7 / BLBP / MRG

Cat. Num. AAA246968

• Gene Names: FABP7; MRG; BLBP; FABPB; B-FABP; LTR2-FABP7
• Reactivity: Bovine, Horse, Human, Monkey, Mouse, Opossum
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A Purified
• Synonyms: FABP7 / BLBP / MRG; Polyclonal Antibody; Rabbit Polyclonal to Human FABP7 / BLBP / MRG; Anti-FABP7 / BLBP / MRG Antibody (aa80-130) IHC-plus; FABP7; B-FABP; BLBP; Brain lipid binding protein; Brain lipid-binding protein; FABPB; Fatty acid-binding protein 7; MRG; Human FABP7; anti-FABP7 antibody

• Host: Rabbit
• Reactivity: Bovine, Horse, Human, Monkey, Mouse, Opossum
• Clonality: Polyclonal
• Specificity: Human FABP7 / BLBP
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 0.05% BSA, 0.05% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 132

• Applicable Applications for anti-FABP7 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 - 10 ug/ml), WB (2 - 4 ug/ml)
• Target Species: Human
• Antigen Type: Protein fragment
• Immunogen Species: FABP7 / BLBP / MRG antibody was raised against Human
• Immunogen: FABP7 / BLBP / MRG antibody was raised against a portion of amino acids 80-130 of human FABP7.
• Epitope: aa80-130
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Immunohistochemistry (IHC)

(Anti-FABP7 / BLBP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using at 3 ug/ml, 2 ug/ml, and 2 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test.)

Related Product Information for anti-FABP7 antibody

FABP7 encodes the brain fatty acid binding protein. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

NCBI and Uniprot Product Information

• NCBI GI #: 4557585
• NCBI GeneID: 2173
• NCBI Accession #: NP_001437.1
• NCBI GenBank Nucleotide #: NM_001446.3
• UniProt Accession #: O15540; O14951; Q6IAU7; Q9H047; B2R4L1
• NCBI Official Full Name: fatty acid-binding protein, brain
• NCBI Official Synonym Full Names: fatty acid binding protein 7, brain
• NCBI Official Symbol: FABP7
• NCBI Official Synonym Symbols: MRG; BLBP; FABPB; B-FABP; LTR2-FABP7
• NCBI Protein Information: fatty acid-binding protein, brain; brain lipid binding protein; brain lipid-binding protein; fatty acid-binding protein 7; Hypothetical protein DKFZp547J2313; brain-type fatty acid-binding protein; mammary-derived growth inhibitor related; mammary-derived growth inhibitor-related
• UniProt Protein Name: Fatty acid-binding protein, brain
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP7
• UniProt Synonym Gene Names: BLBP; FABPB; MRG; BLBP; B-FABP
• UniProt Entry Name: FABP7_HUMAN

NCBI Description

The protein encoded by this gene is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP7: B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding; Cell cycle regulation; Cell development/differentiation

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: cell projection; cell soma; cytoplasm; nucleus

Molecular Function: transporter activity; lipid binding

Biological Process: nervous system development; negative regulation of cell proliferation; epithelial cell proliferation; prepulse inhibition; neurogenesis; transport; cell proliferation in forebrain