Rabbit KIR4.1 Polyclonal Antibody | anti-KIR4.1 antibody

KIR4.1 polyclonal antibody

Cat. Num. AAA3014677

• Gene Names: KCNJ10; KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: KIR4.1; Polyclonal Antibody; KIR4.1 polyclonal antibody; ATP-sensitive inward rectifier potassium channel 10; ATP-dependent inwardly rectifying potassium channel Kir4.1; Inward rectifier K(+) channel Kir1.2; Potassium channel; inwardly rectifying subfamily J member 10; KCNJ10; anti-KIR4.1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KIR4.1 polyclonal antibody detects endogenous levels of KIR4.1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KIR4.1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:2000; IHC: 1:50~1:100
• Immunogen: Synthetic peptide, corresponding to Human KIR41
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of KIR4.1 polyclonal antibody at 1:2000 dilutionLane1:HEK293T whole cell lysate(40ug)Lane2: The Kidney tissue lysate of Rat(40ug)Lane3: The Brain tissue lysate of Mouse(40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human Kidney cancer tissue using KIR4.1 antibody at dilution of 1:20 (x40 lens).)

Related Product Information for anti-KIR4.1 antibody

The KIR (for inwardly rectifying potassium channel) family of potassium channels possess a greater tendency to allow potassium to flow into the cell rather than out of it. KIR4.1, also known as Kir1.2, is highly expressed in brain including glial cells, astrocytes and cortical neurons. KIR4.1 is also expressed in myelin-synthesizing oligodendrocytes and is crucial to myelination in the developing nervous system. The gene encoding human KIR4.1 maps to chromosome 1. KIR4.2, also known as Kir1.3, is expressed in kidney, lung, heart, thymus and thyroid during development. The gene encoding human KIR4.2 maps to chromosome 21 in the Down syndrome chromosome region 1, and KIR4.2 may play a role in the pathogenesis of Down's syndrome. KIR5.1 forms functional channels only by coexpression with either KIR4.1 or KIR4.2 in the kidney and pancreas.

NCBI and Uniprot Product Information

• NCBI GI #: 25121966
• NCBI GeneID: 3766
• NCBI Accession #: NP_002232.2
• NCBI GenBank Nucleotide #: NM_002241.4
• UniProt Accession #: P78508; Q5VUT9; Q8N4I7; Q92808; A3KME7
• Molecular Weight: 42,508 Da
• NCBI Official Full Name: ATP-sensitive inward rectifier potassium channel 10
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily J member 10
• NCBI Official Symbol: KCNJ10
• NCBI Official Synonym Symbols: KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN
• NCBI Protein Information: ATP-sensitive inward rectifier potassium channel 10
• UniProt Protein Name: ATP-sensitive inward rectifier potassium channel 10
• UniProt Gene Name: KCNJ10

NCBI Description

This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]

Uniprot Description

May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (). In the kidney, together with KCNJ16, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules.