Rabbit FABP5 Polyclonal Antibody | anti-FABP5 antibody

FABP5 Antibody

Cat. Num. AAA9603343

• Gene Names: FABP5; EFABP; KFABP; E-FABP; PAFABP; PA-FABP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: FABP5; Polyclonal Antibody; FABP5 Antibody; CFABP; Cutaneous fatty acid binding protein; DA11; Differentiation associated lipid binding protein LP2; E-FABP; EFABP; Epidermal-type fatty acid-binding protein; FABP5_HUMAN; fatty acid binding protein 5 (psoriasis-associated); Fatty acid binding protein epidermal; Fatty acid-binding protein 5; Fatty acid-binding protein; epidermal; Keratinocyte lipid binding protein; KFABP; Klbp; mal1; PA-FABP; PAFABP; Psoriasis associated fatty acid binding protein homolog; Psoriasis-associated fatty acid-binding protein homolog; anti-FABP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FABP5 antibody detects endogenous levels of FABP5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 135

• Applicable Applications for anti-FABP5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human FABP5.
• Subcellular Location: Cytoplasm.
• Tissue Specificity: Keratinocytes; highly expressed in psoriatic skin.
• Predicted Cross Reactivity: Bovine, Horse, Sheep, Rabbit
• Similarity: Bovine (92%), Horse (92%), Sheep (92%), Rabbit (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603343 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of A375 cells, using FABP5 antibody.)

Related Product Information for anti-FABP5 antibody

Function: High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
Similarity: Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

NCBI and Uniprot Product Information

• NCBI GI #: 4557581
• NCBI GeneID: 2171
• NCBI Accession #: NP_001435.1
• NCBI GenBank Nucleotide #: NM_001444.2
• UniProt Accession #: Q01469; B2R4K0
• Molecular Weight: Observed: 15 kDa; Predicted: 16 kDa
• NCBI Official Full Name: fatty acid-binding protein 5
• NCBI Official Synonym Full Names: fatty acid binding protein 5
• NCBI Official Symbol: FABP5
• NCBI Official Synonym Symbols: EFABP; KFABP; E-FABP; PAFABP; PA-FABP
• NCBI Protein Information: fatty acid-binding protein 5
• UniProt Protein Name: Fatty acid-binding protein 5
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP5
• UniProt Synonym Gene Names: E-FABP1 PublicationManual assertion based on opinion iniRef.9; PA-FABP

NCBI Description

This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus.[provided by RefSeq, Feb 2011]

Uniprot Description

Intracellular carrier for long-chain fatty acids and related active lipids, such as the endocannabinoid, that regulates the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors (PubMed:22170058). Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta; which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation (). May be involved in keratinocyte differentiation (PubMed:8092987).