SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
DDT recombinant protein
DDT, 1-118aa, Human, His tag, E Coli
Gene Names
DDT; DDCT
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
DDT; 1-118aa; Human; His tag; E Coli; D-dopachrome tautomerase; DDCT; D-dopachtome decarboxylase; D dopachrome decarboxylase; D dopachrome tautomerase; Phenylpyruvate tautomerase II; ; DDT recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
Sequence Length
118
Applicable Applications for DDT recombinant protein
SDS-PAGE
Tag Information
His-tag
Domain
1-118aa
Preparation and Storage
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for DDT recombinant protein
DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine. Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for DDT recombinant protein
References
Arisawa K., et al. (2009) Toxicology. 255 (1-2):6-14.; Nishihira J., et al. (1999) Biochemistry. 38 (11):3268-79.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14.8 kDa (138aa), confirmed by MALDI-TOF
NCBI Official Full Name
D-dopachrome decarboxylase
NCBI Official Synonym Full Names
D-dopachrome tautomerase
NCBI Official Symbol
DDT
NCBI Official Synonym Symbols
DDCT
NCBI Protein Information
D-dopachrome decarboxylase
UniProt Protein Name
D-dopachrome decarboxylase
Protein Family
UniProt Gene Name
DDT
NCBI Description
D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]
Uniprot Description
Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
Research Articles on DDT
Similar Products
Product Notes
The DDT ddt (Catalog #AAA203497) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag!!Domain||1-118aa. AAA Biotech's DDT can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DDT ddt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL. It is sometimes possible for the material contained within the vial of "DDT, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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