Rabbit FABP2 Polyclonal Antibody | anti-FABP2 antibody

Anti-FABP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103426

• Gene Names: Fabp2; Fabpi; I-FABP
• Reactivity: Mouse; Predicted to React with: Rat, Bovine (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: FABP2; Polyclonal Antibody; Anti-FABP2 Antibody; Rabbit Polyclonal; FABP2 Antibody; Rabbit PAb; Antigen Affinity Purified; fatty acid binding protein 2; Anti-Fabpi Antibody; Anti-I-FABP Antibody; anti-FABP2 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Rat, Bovine (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse FABP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-FABP2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Mouse FABP2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse FABP2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-FABP2 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse small intestine tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of FABP2 in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FABP2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-FABP2 antibody

Fatty acid binding protein (FABP) is one of the intracellular proteins, with a low molecular weight of approximately 15 kDa, that plays important roles in the transportation and metabolism of long-chain fatty acids. FABP family proteins could be used as tissue specific injury marker based on the following characteristics of FABP. The intestinal fatty acid binding protein (I-FABP), or fatty acid-binding protein 2 (FABP2), an intracellular protein expressed only in the intestine, involved in the absorption and intracellular transport of dietary long chain fatty acids. The FABP2 gene is proposed as a candidate gene for diabetes because the protein it codes is involved in fatty acid (FA) absorption and metabolism. Numerous studies have assessed FABP2 gene variants. A transition of G to A at codon 54 of FABP2 results in an amino acid substitution (Ala54 to Thr54), which is common in diverse populations and results in increased FA absorption in vivo. Some evidence indicates that this variant may be associated with type 2 diabetes. This polymorphism was associated with some cardiovascular risk factors. The cytosolic human intestinal fatty acid binding protein (hFABP2) is proposed to be involved in intestinal absorption of long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

References

de Luis DA, et al. (2007) Influence of ALA54THR polymorphism of fatty acid-binding protein 2 on obesity and cardiovascular risk factors. Horm Metab Res. 39(11): 830-4.Klapper M, et al.. (2007) The human intestinal fatty acid binding protein (hFABP2) gene is regulated by HNF-4alpha. Biochem Biophys Res Commun. 356(1): 147-52.

NCBI and Uniprot Product Information

• NCBI GI #: 6679737
• NCBI GeneID: 14079
• NCBI Accession #: NP_032006.1
• NCBI GenBank Nucleotide #: NM_007980.3
• UniProt Accession #: P55050
• Molecular Weight: 15,126 Da
• NCBI Official Full Name: fatty acid-binding protein, intestinal
• NCBI Official Synonym Full Names: fatty acid binding protein 2, intestinal
• NCBI Official Symbol: Fabp2
• NCBI Official Synonym Symbols: Fabpi; I-FABP
• NCBI Protein Information: fatty acid-binding protein, intestinal
• UniProt Protein Name: Fatty acid-binding protein, intestinal
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: Fabp2
• UniProt Synonym Gene Names: Fabpi; I-FABP

NCBI Description

The protein encoded by this gene is part of the fatty acid binding protein family (FABP). FABPs are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands and participate in fatty acid uptake, transport, and metabolism. This protein functions within enterocytes, possibly to sense lipids as part of energy homeostasis. In humans polymorphisms are associated with increased fat oxidation and insulin resistance. In mice deficiency of this gene alters body weight in a gender-specific manner and causes hyperinsulinemia. [provided by RefSeq, Jan 2013]

Uniprot Description

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.