FABP2 recombinant protein

FABP2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120014

• Gene Names: FABP2; FABPI; I-FABP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FABP2; FABP2 Protein; Human; Recombinant (His Tag); Human FABP2/I-FABP Protein (His Tag); FABPI Protein; I-FABP Protein; MGC133132 Protein; fatty acid binding protein 2; FABP2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp132

• Species: Human
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FABP2 (P12104-1)(Met 1-Asp 132) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FABP2 recombinant protein

Background: Fatty acid binding protein (FABP) is one of the intracellular proteins, with a low molecular weight of approximately 15kDa, that plays important roles in the transportation and metabolism of long-chain fatty acids. FABP family proteins could be used as tissue specific injury marker based on the following characteristics of FABP. The intestinal fatty acid binding protein (I-FABP), or fatty acid-binding protein 2 (FABP2), an intracellular protein expressed only in the intestine, involved in the absorption and intracellular transport of dietary long chain fatty acids. The FABP2 gene is proposed as a candidate gene for diabetes because the protein it codes is involved in fatty acid (FA) absorption and metabolism. Numerous studies have assessed FABP2 gene variants. A transition of G to A at codon 54 of FABP2 results in an amino acid substitution (Ala54 to Thr54), which is common in diverse populations and results in increased FA absorption in vivo. Some evidence indicates that this variant may be associated with type 2 diabetes. This polymorphism was associated with some cardiovascular risk factors. The cytosolic human intestinal fatty acid binding protein (hFABP2) is proposed to be involved in intestinal absorption of long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

References

de Luis DA, et al. (2007) Influence of ALA54THR polymorphism of fatty acid-binding protein 2 on obesity and cardiovascular risk factors. Horm Metab Res. 39(11): 830-4.Klapper M, et al.. (2007) The human intestinal fatty acid binding protein (hFABP2) gene is regulated by HNF-4alpha. Biochem Biophys Res Commun. 356(1): 147-52.

NCBI and Uniprot Product Information

• NCBI GI #: 119805
• NCBI GeneID: 2169
• UniProt Accession #: P12104; Q2NKJ1
• Molecular Weight: 132
• NCBI Official Full Name: Fatty acid-binding protein, intestinal
• NCBI Official Synonym Full Names: fatty acid binding protein 2, intestinal
• NCBI Official Symbol: FABP2
• NCBI Official Synonym Symbols: FABPI; I-FABP
• NCBI Protein Information: fatty acid-binding protein, intestinal; fatty acid-binding protein 2; intestinal-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, intestinal
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP2
• UniProt Synonym Gene Names: FABPI; I-FABP
• UniProt Entry Name: FABPI_HUMAN

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP2: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Chromosomal Location of Human Ortholog: 4q28-q31

Cellular Component: cytoplasm

Molecular Function: transporter activity; fatty acid binding

Biological Process: transport; digestion