Rabbit F1A alpha Polyclonal Antibody | anti-FEM1B antibody

F1A alpha Antibody

Cat. Num. AAA150640

• Gene Names: FEM1B; F1AA; F1A-ALPHA; FEM1-beta
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: F1A alpha Antibody is affinity chromatography purified via peptide column.
• Synonyms: F1A alpha; Polyclonal Antibody; F1A alpha Antibody; F1AA; F1A-ALPHA; FEM1-beta; KIAA0396; Protein fem-1 homolog B; FEM1b; fem-1 homolog b (C. elegans); anti-FEM1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: F1A alpha Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 627

• Applicable Applications for anti-FEM1B antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: F1Ab antibody can be used for detection of F1Ab by Western blot at 1 mug/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: F1A antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A.
• Buffer: F1A alpha Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: F1A alpha antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 μg/ml.Green: F1A alpha Antibody (2279)Blue: DAPI staining)

Related Product Information for anti-FEM1B antibody

F1A alpha Antibody: Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1β. F1Aalpha/FEM1β is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 6175869
• NCBI GeneID: 10116
• UniProt Accession #: Q9UK73; O43146
• Molecular Weight: 70 kDa
• NCBI Official Full Name: FEM-1-like death receptor binding protein
• NCBI Official Synonym Full Names: fem-1 homolog b (C. elegans)
• NCBI Official Symbol: FEM1B
• NCBI Official Synonym Symbols: F1AA; F1A-ALPHA; FEM1-beta
• NCBI Protein Information: protein fem-1 homolog B; FEM-1-like death receptor binding protein; fem-1-like in apoptotic pathway protein alpha; fem-1-like death receptor-binding protein alpha
• UniProt Protein Name: Protein fem-1 homolog B
• UniProt Gene Name: FEM1B
• UniProt Synonym Gene Names: F1AA; KIAA0396; FEM1b; F1A-alpha
• UniProt Entry Name: FEM1B_HUMAN

NCBI Description

This gene encodes an ankyrin repeat protein that belongs to the death receptor-associated family of proteins and plays a role in mediating apoptosis. The encoded protein is also thought to function in the replication stress-induced checkpoint signaling pathway via interaction with checkpoint kinase 1. [provided by RefSeq, Aug 2013]

Uniprot Description

FEM1B: Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1. Belongs to the fem-1 family.

Protein type: Cell development/differentiation; Ligase; Ubiquitin conjugating system; Apoptosis

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; death receptor binding; ubiquitin-protein ligase activity

Biological Process: regulation of ubiquitin-protein ligase activity; apoptosis; protein ubiquitination