Immunohistochemistry (IHC)
(MBS9602106 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse ETL Polyclonal Antibody | anti-ETL antibody
ETL Antibody
Gene Names
ADGRL4; ETL; ELTD1; KPG_003
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ETL; Polyclonal Antibody; ETL Antibody; ADGRL4; Adhesion G protein-coupled receptor L4; EGF; EGF latrophilin and seven transmembrane domain containing 1; EGF TM7 latrophilin related protein; latrophilin and seven transmembrane domain-containing protein 1; latrophilin; and 7 transmembrane domain-containing protein 1; EGF-; TM7-; and latrophilin-related protein; EGF-TM7-latrophilin-related protein; EGFTM7 latrophilin related protein; ELTD 1; ELTD1; ELTD1_HUMAN; ETL protein; KPG_003; UNQ202/PRO228; zgc:63629; anti-ETL antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ETL antibody detects endogenous levels of total ETL
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
690
Applicable Applications for anti-ETL antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human ETL
Subcellular Location
Cell Membrane
Tissue Specificity
Detected in the majority of epithelial cells in tumor and normal tissues. Expressed also in human umbilical vein endothelial cells.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9602106 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602106 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of ETL using JK whole cell lysates)
Western Blot (WB)
(Western blot analysis of ETL using JK whole cell lysates)
Related Product Information for anti-ETL antibody
Function: Endothelial orphan receptor that acts as a key regulator of angiogenesis.
Subunit Structure: Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked.
Post-translational Modifications: Glycosylated. Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
Similarity: The transmembrane domain is not required for cleavage, but it is required for dimer formation. Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Subunit Structure: Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked.
Post-translational Modifications: Glycosylated. Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
Similarity: The transmembrane domain is not required for cleavage, but it is required for dimer formation. Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 77 kDa
Predicted: 78 kDa
Predicted: 78 kDa
NCBI Official Full Name
adhesion G protein-coupled receptor L4
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor L4
NCBI Official Symbol
ADGRL4
NCBI Official Synonym Symbols
ETL; ELTD1; KPG_003
NCBI Protein Information
adhesion G protein-coupled receptor L4
UniProt Protein Name
Adhesion G protein-coupled receptor L4
UniProt Gene Name
ADGRL4
UniProt Synonym Gene Names
ELTD1; ETL; ETL protein
Uniprot Description
Endothelial orphan receptor that acts as a key regulator of angiogenesis.
Research Articles on ETL
Similar Products
Product Notes
The ETL adgrl4 (Catalog #AAA9602106) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ETL Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ETL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000 IHC: 1:200. Researchers should empirically determine the suitability of the ETL adgrl4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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