Rabbit anti-Human ERVWE1/HERV/Synctyin Polyclonal Antibody | anti-ERVW-1 antibody

Anti-ERVWE1/HERV/Synctyin Antibody (aa400-429)

Cat. Num. AAA2401803

• Gene Names: ERVW-1; ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Ammonium sulfate precipitation
• Synonyms: ERVWE1/HERV/Synctyin; Polyclonal Antibody; Anti-ERVWE1/HERV/Synctyin Antibody (aa400-429); Rabbit Polyclonal to Human Endogenous Retrovirus ERVWE1/HERV/Synctyin; ERVW-1; Env-W; Envelope polyprotein gPr73; ERVWE1; HERV-W-ENV; Envelope glycoprotein; Enverin; ENVW; HERV-7q Envelope protein; HERV-W Env glycoprotein; HERV-W envelope protein; HERVW; Syncytin A; Syncytin-1; env; Envelope protein; HERV-7q; HERV-W; HERV7Q; HERVWENV; Syncytin; anti-ERVW-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This HERV (ERVWE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from the Central region of human HERV (ERVWE1).
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 538

• Applicable Applications for anti-ERVW-1 antibody: Immunohistochemistry (IHC), Immunohistochemistry (IHC-P) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1:100; WB: 1:1000; ; Optimal dilution to be determined by researcher.
• Target: ERVWE1/ HERV / Syncytin
• Immunogen Species: ERVWE1/ HERV / Syncytin antibody was raised againist Human
• Epitope: aa400-429
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Related Product Information for anti-ERVW-1 antibody

human endogenous retrovirus (HERV) families are expressed in normalplacental tissue at high levels, suggesting that HERVs are functionallyimportant in reproduction. This gene is part of an HERV provirus onchromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have beenselectively preserved.

NCBI and Uniprot Product Information

• NCBI GI #: 48949851
• NCBI GeneID: 30816
• NCBI Accession #: NP_055405.3
• NCBI GenBank Nucleotide #: NM_014590.3
• UniProt Accession #: Q9UQF0; O95244; O95245; Q8NHY7; Q9NRZ2; Q9NZG3; B2RPD4
• NCBI Official Full Name: syncytin-1
• NCBI Official Synonym Full Names: endogenous retrovirus group W member 1, envelope
• NCBI Official Symbol: ERVW-1
• NCBI Official Synonym Symbols: ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV
• NCBI Protein Information: syncytin-1
• UniProt Protein Name: Syncytin-1
• Protein Family: Syncytin
• UniProt Gene Name: ERVW-1
• UniProt Synonym Gene Names: ERVWE1; SU; TM

NCBI Description

Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. May induce fusion through binding of SLC1A4 and SLC1A5 (PubMed:10708449, PubMed:12050356, PubMed:23492904).