Rabbit anti-Human, Mouse ERO1LB Polyclonal Antibody | anti-ERO1LB antibody

Rabbit Polyclonal to Human ERO1LB

Cat. Num. AAA248127

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Immunoaffinity Purified
• Synonyms: ERO1LB; Polyclonal Antibody; Rabbit Polyclonal to Human ERO1LB; Anti-ERO1LB Antibody (aa274-304) IHC-plus; ERO1-L(beta); ERO1-like protein beta; ERO1-like beta (S. cerevisiae); ERO1-L-beta; Oxidoreductin-1-L-beta; Human ERO1LB; anti-ERO1LB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Human ERO1LB
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 467

• Applicable Applications for anti-ERO1LB antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:10 - 1:50), IHC-P (1:50 - 1:100), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH-conjugated synthetic peptide from C-terminal region of human ERO1LB.
• Immunogen Type: Synthetic peptide
• Immunogen: ERO1LB antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human ERO1LB.
• Antigen Modification: aa274-304
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-ERO1LB antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(ERO1LB Antibody (C-term) IHC of formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This demonstrates the use of the ERO1LB Antibody (C-term) for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(ERO1LB Antibody (C-term) flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(ERO1LB Antibody (C-term) Western blot of MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the ERO1LB antibody detected the ERO1LB protein (arrow).)

NCBI and Uniprot Product Information

• NCBI GI #: 116241353
• NCBI GeneID: 56605
• Molecular Weight: 16,096 Da
• NCBI Official Full Name: ERO1-like protein beta
• NCBI Official Synonym Full Names: ERO1-like beta (S. cerevisiae)
• NCBI Official Symbol: ERO1LB
• NCBI Protein Information: ERO1-like protein beta; ERO1-L-beta; oxidoreductin-1-L-beta; endoplasmic oxidoreductin-1-like protein B; endoplasmic reticulum oxidoreductin-1-like protein B
• UniProt Protein Name: ERO1-like protein beta
• UniProt Gene Name: ERO1LB
• UniProt Synonym Gene Names: ERO1-L-beta
• UniProt Entry Name: ERO1B_HUMAN

Uniprot Description

ERO1LB: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress. Belongs to the EROs family.

Protein type: EC 1.8.4.-; Oxidoreductase; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1q42.2-q43

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum

Molecular Function: protein binding; unfolded protein binding; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor

Biological Process: insulin processing; extracellular matrix organization and biogenesis; protein maturation via protein folding; 4-hydroxyproline metabolic process; protein folding; cell redox homeostasis; glucose homeostasis