Rabbit anti-Human ERK5 Polyclonal Antibody | anti-ERK5 antibody

ERK5, CT (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7) (MaxLight 750)

Cat. Num. AAA6474854

• Gene Names: MAPK7; BMK1; ERK4; ERK5; PRKM7
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: ERK5; Polyclonal Antibody; CT (Extracellular Signal-regulated Kinase 5; ERK-5; Big MAP Kinase 1; BMK1; BMK-1; Mitogen-activated Protein Kinase 7; MAP Kinase 7; MAPK 7; MAPK7; PRKM7) (MaxLight 750); EC=2.7.11.24; anti-ERK5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ERK5.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-ERK5 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 776-805 in the C-terminal region of human ERK5. Species sequence homology: mouse, rat, bovine
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of K562 cell line lysates (35ug/lane) usingMBS625993. ERK5 (arrow) was detected usingMBS625993.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS625993, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS625993 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-ERK5 antibody

Antibodies; Protein Kinases

References

1. Zhou, G., et al., J. Biol. Chem. 270(21): 12, 665-12, 669 (1995). 2. Lee, J.D., et al., Biochem. Biophys. Res. Commun. 213(2): 715-724 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 20986497
• NCBI GeneID: 5598
• NCBI Accession #: NP_002740.2
• NCBI GenBank Nucleotide #: NM_002749.3
• UniProt Accession #: Q13164; Q16634; Q59F50; Q6QLU7; Q7L4P4; Q969G1; Q96G51
• Molecular Weight: 88,386 Da
• NCBI Official Full Name: mitogen-activated protein kinase 7 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 7
• NCBI Official Symbol: MAPK7
• NCBI Official Synonym Symbols: BMK1; ERK4; ERK5; PRKM7
• NCBI Protein Information: mitogen-activated protein kinase 7; BMK-1; ERK-5; MAPK 7; BMK1 kinase; MAP kinase 7; big MAP kinase 1; extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase 7
• UniProt Gene Name: MAPK7
• UniProt Synonym Gene Names: BMK1; ERK5; PRKM7; MAP kinase 7; MAPK 7; BMK-1; ERK-5
• UniProt Entry Name: MK07_HUMAN