Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EREG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Rabbit EREG Polyclonal Antibody | anti-EREG antibody
EREG Rabbit pAb
Customer Validation: WB: Mouse
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EREG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EREG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
NCBI and Uniprot Product Information
Uniprot Description
Epiregulin: Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: extracellular region; extracellular space; integral to plasma membrane
Molecular Function: epidermal growth factor receptor binding; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity
Biological Process: anatomical structure morphogenesis; cell-cell signaling; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; female meiosis; keratinocyte differentiation; keratinocyte proliferation; luteinizing hormone signaling pathway; MAPKKK cascade; mRNA transcription; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent; oocyte maturation; organ morphogenesis; ovarian cumulus expansion; ovulation; phosphoinositide-mediated signaling; positive regulation of cell proliferation; positive regulation of cytokine biosynthetic process; positive regulation of cytokine production; positive regulation of DNA replication; positive regulation of epidermal growth factor receptor activity; positive regulation of fibroblast proliferation; positive regulation of innate immune response; positive regulation of interleukin-6 biosynthetic process; positive regulation of mitosis; positive regulation of phosphorylation; positive regulation of protein kinase activity; positive regulation of smooth muscle cell proliferation; primary follicle stage, oogenesis; regulation of phosphoinositide 3-kinase cascade; wound healing
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Product Notes
The EREG ereg (Catalog #AAA9142291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EREG Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EREG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000 Customer Validation: WB: Mouse. Researchers should empirically determine the suitability of the EREG ereg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GVRCEHFFLT VHQPLSKEYV ALTVILIILF LITVVGSTYY FCRWYRNRKS KEPKKEYERV TSGDPELPQV. It is sometimes possible for the material contained within the vial of "EREG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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