Rabbit Ankyrin repeat and SOCS box-containing protein Polyclonal Antibody | anti-ASB2 antibody

Ankyrin repeat and SOCS box-containing protein (Asb-2)

Cat. Num. AAA613477

• Gene Names: ASB2; ASB-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Ankyrin repeat and SOCS box-containing protein; Polyclonal Antibody; Ankyrin repeat and SOCS box-containing protein (Asb-2); Anti -Ankyrin repeat and SOCS box-containing protein (Asb-2); anti-ASB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Should cross react with mouse and rat Asb-2, based on amino acid sequences used.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-ASB2 antibody: Western Blot (WB)
• Application Notes: Western blot analysis of Asb-2 using antibody at 1:1,000 dilution against 15 ug/lane of human spleen lysate (lane 1) and lung lysate (lane 2).; Dilution: Western blot: 1:500-1,000
• Immunogen: Synthetic peptides containing amino acids 75-93 and 348-368 of human Asb-2 protein.
• Preparation and Storage: It is supplied as Protein G purified Ig fraction. May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(estern blot analysis of Asb-2 using IMG-502 at 1:1,000 dilution against 15 ug/lane of human spleen lysate (lane 1) and lung lysate (lane 2).)

Related Product Information for anti-ASB2 antibody

The ankyrin repeat and SOCS box-containing (Asb) protein family is comprised of members which each contain "SOCS boxes" but differ from other families of protein in the motifs they contain upstream of the SOCS box(1). SOCS boxes are carboxy-terminal regions of homology found in the suppressor of cytokine signaling family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases. Asb-2 has been found to induce growth inhibition and chromatin condensation in myeloid leukemia cells(2).

Product Categories/Family for anti-ASB2 antibody

Antibodies; Abs to SOCS

NCBI and Uniprot Product Information

• NCBI GI #: 289666524
• NCBI GeneID: 51676
• UniProt Accession #: Q96Q27; Q9NSU5; Q9Y567; B2RDP9; B4E166
• Molecular Weight: 65,084 Da
• NCBI Official Full Name: ankyrin repeat and SOCS box-containing protein 2a
• NCBI Official Synonym Full Names: ankyrin repeat and SOCS box containing 2
• NCBI Official Symbol: ASB2
• NCBI Official Synonym Symbols: ASB-2
• NCBI Protein Information: ankyrin repeat and SOCS box protein 2; ankyrin repeat and SOCS box-containing protein 2a
• UniProt Protein Name: Ankyrin repeat and SOCS box protein 2
• Protein Family: Anthranilate synthase
• UniProt Gene Name: ASB2
• UniProt Synonym Gene Names: ASB-2
• UniProt Entry Name: ASB2_HUMAN

NCBI Description

This gene encodes a member of the ankyrin repeat and SOCS box-containing (ASB) protein family. These proteins play a role in protein degradation by coupling suppressor of cytokine signalling (SOCS) proteins with the elongin BC complex. The encoded protein is a subunit of a multimeric E3 ubiquitin ligase complex that mediates the degradation of actin-binding proteins. This gene plays a role in retinoic acid-induced growth inhibition and differentiation of myeloid leukemia cells. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ASB2: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 14q31-q32

Cellular Component: Cul5-RING ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: myoblast differentiation; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; signal transduction