Rabbit ERCC1 Polyclonal Antibody | anti-ERCC1 antibody

ERCC1 (Excision Repair Cross Complement Group 1, UV20, COFS4, RAD10) (HRP)

Cat. Num. AAA6416204

• Gene Names: ERCC1; UV20; COFS4; RAD10
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: ERCC1; Polyclonal Antibody; ERCC1 (Excision Repair Cross Complement Group 1; UV20; COFS4; RAD10) (HRP); anti-ERCC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ERCC1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ERCC1 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human ERCC1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ERCC1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using ERCC1 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney cancer using ERCC1 antibody at dilution of 1:100 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using ERCC1 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-ERCC1 antibody

Antibodies; DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 260593725
• NCBI GeneID: 2067
• NCBI Accession #: NP_001159521.1
• NCBI GenBank Nucleotide #: NM_001166049.1
• UniProt Accession #: P07992; Q7Z7F5; Q96S40
• Molecular Weight: 32,562 Da
• NCBI Official Full Name: DNA excision repair protein ERCC-1 isoform 3
• NCBI Official Synonym Full Names: excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
• NCBI Official Symbol: ERCC1
• NCBI Official Synonym Symbols: UV20; COFS4; RAD10
• NCBI Protein Information: DNA excision repair protein ERCC-1
• UniProt Protein Name: DNA excision repair protein ERCC-1
• Protein Family: DNA excision repair protein
• UniProt Gene Name: ERCC1
• UniProt Entry Name: ERCC1_HUMAN

NCBI Description

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Uniprot Description

Function: Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.

Subunit structure: Heterodimer composed of ERCC1 and XPF/ERRC4.

Subcellular location: Nucleus.

Involvement in disease: Cerebro-oculo-facio-skeletal syndrome 4 (COFS4) [MIM:610758]: A disorder of prenatal onset characterized by microcephaly, congenital cataracts, facial dysmorphism, neurogenic arthrogryposis, growth failure and severe psychomotor retardation. COFS is considered to be part of the nucleotide-excision repair disorders spectrum that include also xeroderma pigmentosum, trichothiodystrophy and Cockayne syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities: Belongs to the ERCC1/RAD10/SWI10 family.