Rabbit EPS15 Polyclonal Antibody | anti-EPS15 antibody

EPS15 Antibody

Cat. Num. AAA9612535

• Gene Names: EPS15; AF1P; AF-1P; MLLT5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: EPS15; Polyclonal Antibody; EPS15 Antibody; AF 1P; AF 1p protein; AF1P; ALL1 fused gene from chromosome 1; Epidermal growth factor receptor pathway substrate 15; Epidermal growth factor receptor substrate 15; EPS 15; MLLT 5; MLLT5; Protein Eps 15; Protein Eps15; anti-EPS15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: EPS15 Antibody detects endogenous levels of total EPS15.; Tissue Specificity: Ubiquitously expressed.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-EPS15 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human EPS15.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.Ubiquitinated.
• Subunit Structure: Interacts with SGIP1. Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2. Interacts (via UIM repeats) with CORO7 (when ubiquitinated at 'Lys-472'). Interacts (via UIM domains) with UBQLN1 (via ubiquitin-like domain) and can interact with both the ubiquitinated and the non-ubiquitinated forms of UBQLN1. Interacts with UBQLN2 (By similarity). (Microbial infection) Interacts with vaccinia virus protein A36.
• Similarity: The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.The UIM (ubiquitin-interacting motif) repeats specifically bind 'Lys-33'-linked ubiquitin.
• Subcellular Location: Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Clathrin-coated pit.; Note: Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes.Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.; Note: Colocalizes with HGS on bilayered clathrin coats on endosomes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-EPS15 antibody

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

NCBI and Uniprot Product Information

• NCBI GI #: 229577185
• NCBI GeneID: 2060
• NCBI Accession #: NP_001153441.1
• NCBI GenBank Nucleotide #: NM_001159969.1
• UniProt Accession #: P42566; Q5SRH4; B2R8J7; D3DPJ2
• Molecular Weight: 98,656 Da
• NCBI Official Full Name: epidermal growth factor receptor substrate 15 isoform B
• NCBI Official Synonym Full Names: epidermal growth factor receptor pathway substrate 15
• NCBI Official Symbol: EPS15
• NCBI Official Synonym Symbols: AF1P; AF-1P; MLLT5
• NCBI Protein Information: epidermal growth factor receptor substrate 15; protein AF-1p; ALL1 fused gene from chromosome 1
• UniProt Protein Name: Epidermal growth factor receptor substrate 15
• Protein Family: Epidermal growth factor receptor substrate
• UniProt Gene Name: EPS15
• UniProt Synonym Gene Names: AF1P; Protein Eps15
• UniProt Entry Name: EPS15_HUMAN

NCBI Description

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009]

Uniprot Description

EPS15: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits. Interacts with SGIP1. Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM1 or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Ubiquitously expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Oncoprotein; Vesicle

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: intracellular membrane-bound organelle; membrane; early endosome membrane; cytoplasm; plasma membrane; AP-2 adaptor complex; coated pit; cytosol

Molecular Function: identical protein binding; protein binding; calcium ion binding; polyubiquitin binding; SH3 domain binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; clathrin cage assembly; cell proliferation; protein transport; vesicle organization and biogenesis; Golgi to endosome transport; endocytosis; endocytic recycling