Rabbit SBDS Polyclonal Antibody | anti-SBDS antibody

SBDS Polyclonal Antibody

Cat. Num. AAA3011461

• Gene Names: SBDS; SDS; SWDS; CGI-97
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SBDS; Polyclonal Antibody; SBDS Polyclonal Antibody; Ribosome maturation protein SBDS; Shwachman-Bodian-Diamond syndrome protein; CGI-97; anti-SBDS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SBDS polyclonal antibody detects endogenous levels of SBDS protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 250

• Applicable Applications for anti-SBDS antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human SBDS.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SBDS polyclonal antibody)

Related Product Information for anti-SBDS antibody

The 249 amino acid Shwachman-Bodian-Diamond syndrome (SBDS) protein belongs to the UPF0023 family. SBDS is widely expressed and may be involved in RNA metabolism. SBDS contains a C-terminal domain, a central domain and an N-terminal domain. The C-terminal domain has a ferredoxin-like fold and is structurally homologous with known RNA-binding domains. The central domain contains a three-helical bundle. The N-terminal domain consists of a three-dimensional a/b fold and is the most frequent target of disease-linked mutations. Mutations in the SBDS gene cause Shwachman-Diamond syndrome (SDS), an autosomal recessive marrow failure disorder marked by hematologic dysfunction, skeletal abnormalities and pancreatic exocrine insufficiency. SDS is also characterized by an increased risk of leukemia and myelodysplasia in as many as one third of affected individuals.

NCBI and Uniprot Product Information

• NCBI GI #: 28416940
• NCBI GeneID: 51119
• NCBI Accession #: NP_057122.2
• NCBI GenBank Nucleotide #: NP_057122.2
• UniProt Accession #: Q9Y3A5
• Molecular Weight: ~ 34kDa
• NCBI Official Full Name: ribosome maturation protein SBDS
• NCBI Official Synonym Full Names: SBDS ribosome maturation factor
• NCBI Official Symbol: SBDS
• NCBI Official Synonym Symbols: SDS; SWDS; CGI-97
• NCBI Protein Information: ribosome maturation protein SBDS
• UniProt Protein Name: Ribosome maturation protein SBDS
• Protein Family: Ribosome maturation protein
• UniProt Gene Name: SBDS
• UniProt Entry Name: SBDS_HUMAN

NCBI Description

This gene encodes a highly conserved protein that plays an essential role in ribosome biogenesis. The encoded protein interacts with elongation factor-like GTPase 1 to disassociate eukaryotic initiation factor 6 from the late cytoplasmic pre-60S ribosomal subunit allowing assembly of the 80S subunit. Mutations within this gene are associated with the autosomal recessive disorder Shwachman-Bodian-Diamond syndrome. This gene has a closely linked pseudogene that is distally located. [provided by RefSeq, Jan 2017]

Uniprot Description

SBDS: Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, triggers the GTP- dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation. Defects in SBDS are the cause of Shwachman-Diamond syndrome (SDS). SDS is an autosomal recessive disorder characterized by pancreatic exocrine insufficiency, hematologic dysfunction, and skeletal abnormalities. Belongs to the SDO1/SBDS family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 7q11.21

Cellular Component: nucleoplasm; spindle pole; cytoplasm; nucleolus; nucleus

Molecular Function: rRNA binding; protein binding; microtubule binding; ribosome binding

Biological Process: bone marrow development; cell proliferation; leukocyte chemotaxis; ribosomal large subunit biogenesis and assembly; mitotic spindle stabilization; inner cell mass cell proliferation; rRNA processing; mature ribosome assembly; bone mineralization

Disease: Shwachman-diamond Syndrome; Aplastic Anemia